Gabius H J, Heil M S, Berger H
Max Planck Institut für experimentelle Medizin, Abteilung Chemie, Göttingen, F.R.G.
Anticancer Res. 1990 Nov-Dec;10(6):1627-31.
Plant lectins have been applied to define the profile of certain carbohydrate determinants in routinely processed sections of specimens from cutaneous neoplasia. To facilitate detection of endogenous receptors with specificity to carbohydrates, termed endogenous lectins, biotinylated neoglycoproteins (glycoproteins derived from chemical glycosylation of a histochemically inert carrier protein) were employed on formalin-fixed, paraffin-embedded sections of 40 cases of cutaneous cancer. These included 20 cases of basal cell epithelioma and 10 cases of squamous cell carcinoma as well as 10 cases of malignant melanoma. A mixed pattern of staining extent was seen for the different types of neoglycoproteins. Glycohistochemical controls ascertained that endogenous lectins with specificity for alpha- or beta-galactosides, alpha-mannosides, alpha-fucosides and alpha-glucosides, respectively, bound the neoglycoproteins. Initial biochemical purification from one case of basal cell epithelioma revealed the molecular characteristics of the endogenous beta-galactoside-specific and alpha-mannoside-specific lectins.
植物凝集素已被应用于确定皮肤肿瘤标本常规处理切片中某些碳水化合物决定簇的概况。为便于检测对碳水化合物具有特异性的内源性受体，即内源性凝集素，在40例皮肤癌的福尔马林固定、石蜡包埋切片上使用了生物素化新糖蛋白（由组织化学惰性载体蛋白化学糖基化衍生而来的糖蛋白）。其中包括20例基底细胞上皮瘤、10例鳞状细胞癌以及10例恶性黑色素瘤。不同类型的新糖蛋白呈现出混合的染色程度模式。糖组织化学对照确定，分别对α-或β-半乳糖苷、α-甘露糖苷、α-岩藻糖苷和α-葡萄糖苷具有特异性的内源性凝集素与新糖蛋白结合。从1例基底细胞上皮瘤中进行的初步生化纯化揭示了内源性β-半乳糖苷特异性和α-甘露糖苷特异性凝集素的分子特征。
