比较甲硫氨酸脑啡肽、白细胞介素 2 和干扰素 γ 对人外周血淋巴细胞亚群的刺激作用。
Comparison of stimulating effect on subpopulations of lymphocytes in human peripheral blood by methionine enkephalin with IL-2 and IFN-γ.
机构信息
Department of Immunology; School of Basic Medical Science; China Medical University, Shenyang, China.
出版信息
Hum Vaccin Immunother. 2012 Aug;8(8):1082-9. doi: 10.4161/hv.20759. Epub 2012 Aug 1.
Abstract
The aim of this study was to investigate the effects of mechanisms of methionine enkephalin (MENK) on lymphocytes in human peripheral blood. We detected CD4+T cells, CD8+T cells, CD4+CD25+ regulatory T cells (Treg), dendritic cells (DCs), natural killer cells (NK), NKT cells and γδT cells before and after treatment with 10 (-12) M MENK, in cell culture by FCM and RT-PCR. Our findings show that MENK stimulating expansion of lymphocyte subpopulationns by inhibiting CD4+CD25+ regulatory T cells (Treg), which is unique discovery of our study. We may use MENK as a drug to treat cancer patients, whose immune systems are damaged by chemotherapy or radiotherapy.
摘要
本研究旨在探讨甲硫氨酸脑啡肽（MENK）对人外周血淋巴细胞作用的机制。我们通过 FCM 和 RT-PCR 检测了细胞培养前后经 10（-12）M MENK 处理后 CD4+T 细胞、CD8+T 细胞、CD4+CD25+调节性 T 细胞（Treg）、树突状细胞（DC）、自然杀伤细胞（NK）、NKT 细胞和 γδT 细胞的变化。我们的研究结果表明，MENK 通过抑制 CD4+CD25+调节性 T 细胞（Treg）来刺激淋巴细胞亚群的扩增，这是我们研究的独特发现。我们可以使用 MENK 作为药物来治疗因化疗或放疗而受损的免疫系统的癌症患者。
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