Park Y, Kim Y S, Kim S I, Kim H, Kim H S
Department of Laboratory Medicine, Yonsei University College of Medicine, Seodaemun-gu, Seoul, South Korea.
Tissue Antigens. 2012 Oct;80(4):376-9. doi: 10.1111/j.1399-0039.2012.01942.x. Epub 2012 Aug 2.
The human leukocyte antigen (HLA)-B51 genotype is one of the well-known genetic factors associated with the development of Behcet's disease. We evaluated three sequence-specific priming (SSP) assays and one real-time PCR assay for detecting HLA-B51 alleles using 93 whole blood samples, which were genotyped by high-resolution sequence-based typing (SBT). All HLA-B51 alleles determined by SBT were detected by the four evaluated assays, and the results for all HLA-B alleles other than HLA-B51 were negative on all assays. Thus, all HLA-B51 tests showed 100% sensitivity and 100% specificity for detecting HLA-B51 alleles. The three SSP assays and the real-time PCR test for HLA-B51 genotyping are simple, but reliable for detecting HLA-B*51 alleles in clinical laboratories.
人类白细胞抗原（HLA）-B51基因型是与白塞病发病相关的著名遗传因素之一。我们使用93份全血样本评估了三种序列特异性引物（SSP）检测方法和一种实时PCR检测方法，以检测HLA-B51等位基因，这些样本通过基于高分辨率序列的分型（SBT）进行基因分型。通过SBT确定的所有HLA-B51等位基因均被这四种评估检测方法检测到，并且所有除HLA-B51之外的HLA-B等位基因在所有检测方法中结果均为阴性。因此，所有HLA-B51检测方法在检测HLA-B51等位基因时均显示出100%的敏感性和100%的特异性。这三种SSP检测方法和用于HLA-B51基因分型的实时PCR检测方法简单，但在临床实验室中检测HLA-B*51等位基因时可靠。
