丙型肝炎病毒感染患者外周血单个核细胞中IFNAR2 mRNA的表达。

Expression of IFNAR2 mRNA in peripheral blood mononuclear cells of patients with HCV infection.

作者信息

Liu Ning, Wen Ying, Sun Cuiming, Liu Pei

机构信息

Department of Infectious Diseases, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning Province, China.

出版信息

Acta Gastroenterol Belg. 2012 Jun;75(2):228-33.

PMID:22870788

Abstract

BACKGROUND

To investigate the differences of interferon-alpha/beta receptor 2 (IFNAR2) mRNA expression level in peripheral blood mononuclear cells (PBMCs) between different stages of hepatitis C virus (HCV) infection and to determine the correlation with the effectiveness of interferon therapy.

METHODOLOGY

58 patients, positive for anti-HCV antibodies, were divided into three groups depending on their clinical symptoms: acute hepatitis (4 cases), chronic hepatitis (46 cases) and liver cirrhosis (8 cases). 15 volunteers served as healthy controls. PBMCs were purified by density gradient centrifugation and IFNAR2 mRNA was amplified from these cells by a reverse transcription-polymerase chain reaction (RT-PCR) assay.

RESULTS

The detection rate of IFNAR2 mRNA was 87.9% (51/58 cases) in the PBMCs of patients with HCV infection, significantly higher than that in the control group (333%, 5/15 cases; P < 0.05). While the positive rate was 93.5% (43/46 cases) in the chronic hepatitis group, which was significantly higher than that in the liver cirrhosis group (50%, 4/8 cases; P < 0.05). Furthermore, there is no significant difference in the positive rates for HCV-RNA of PBMCs among groups (P > 0.05). The positive rate of IFNAR2 mRNA in PBMCs was not correlated with the viral load of HCV-RNA in serum (P > 0.05). However, higher expression of IFNAR2 mRNA in the PBMCs did correlate with the effectiveness of interferon therapy (P < 0.05).

CONCLUSIONS

HCV infection up-regulates the expression of IFNAR2 mRNA in PBMCs. IFNAR2 mRNA expression in the chronic hepatitis group was higher than that in the liver cirrhosis group, and significantly correlated with the effectiveness of interferon therapy, which was independent of the viral load.

摘要

背景

探讨丙型肝炎病毒（HCV）感染不同阶段外周血单个核细胞（PBMC）中α/β干扰素受体2（IFNAR2）mRNA表达水平的差异，并确定其与干扰素治疗效果的相关性。

方法

58例抗HCV抗体阳性患者根据临床症状分为三组：急性肝炎组（4例）、慢性肝炎组（46例）和肝硬化组（8例）。15名志愿者作为健康对照。通过密度梯度离心法纯化PBMC，并采用逆转录-聚合酶链反应（RT-PCR）法从这些细胞中扩增IFNAR2 mRNA。

结果

HCV感染患者PBMC中IFNAR2 mRNA的检出率为87.9%（51/58例），显著高于对照组（33.3%，5/15例；P<0.05）。慢性肝炎组阳性率为93.5%（43/46例），显著高于肝硬化组（50%，4/8例；P<0.05）。此外，各组PBMC中HCV-RNA的阳性率差异无统计学意义（P>0.05）。PBMC中IFNAR2 mRNA的阳性率与血清中HCV-RNA的病毒载量无关（P>0.05）。然而，PBMC中IFNAR2 mRNA的高表达与干扰素治疗效果相关（P<0.05）。