INRA, UMR 1260 Nutriments Lipidiques et Prévention des Maladies Métaboliques, Marseille F-13385, France.
Food Chem. 2012 Oct 15;134(4):2560-4. doi: 10.1016/j.foodchem.2012.04.043. Epub 2012 Apr 17.
The effects of fat-soluble vitamins (such as vitamins A and E) and lipid microconstituents (such as carotenoids) on human health are now well established. However, high-performance liquid chromatography (HPLC) methods able to detect these molecules in simultaneous runs are often difficult to set up. We report here a 35-min reversed-phase HPLC method using a single C30 column kept at 35°C with a gradient system of methanol, methyl-tert-butyl ether and water at a flow-rate of 1 mL/min. This method resolves 11 carotenoids, retinol, α- and γ-tocopherol from complex matrixes such as food samples, human plasma and human adipose tissue within 35 min. The method is also able to separate coenzyme Q(10). The intra-day and inter-day coefficients of variation are suitable for routine clinical and scientific applications for the determination of lipid micronutrients from various sample types.
脂溶性维生素（如维生素 A 和 E）和脂类微量成分（如类胡萝卜素）对人体健康的影响现已得到充分证实。然而，能够在一次运行中同时检测这些分子的高效液相色谱（HPLC）方法往往难以建立。我们在此报告一种 35 分钟的反相 HPLC 方法，使用单根 C30 柱，在 35°C 下，甲醇、甲基叔丁基醚和水的梯度系统以 1 毫升/分钟的流速运行。该方法可在 35 分钟内从食品样品、人血浆和人脂肪组织等复杂基质中分离出 11 种类胡萝卜素、视黄醇、α-和γ-生育酚以及辅酶 Q(10)。该方法还能够分离辅酶 Q(10)。日内和日间变异系数适用于各种样品类型的脂类微量营养素的常规临床和科学应用。
