在无血清无动物成分的限定条件下，从人胚胎干细胞和诱导多能干细胞中可扩展生产可移植的多巴胺能神经元。
Scalable production of transplantable dopaminergic neurons from hESCs and iPSCs in xeno-free defined conditions.
作者信息
Swistowski Andrzej, Zeng Xianmin
机构信息
Buck Institute for Age Research, Novato, California, USA.
出版信息
Curr Protoc Stem Cell Biol. 2012 Aug;Chapter 2:Unit2D.12. doi: 10.1002/9780470151808.sc02d12s22.
Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) are potentially an unlimited cell source for cell replacement therapy and personalized medicine. Before hESC- and iPSC-based therapy can be moved from bench to bedside, however, it is essential to establish protocols for generating therapeutically relevant cells, like dopaminergic neurons in defined conditions that are suitable for scalable good manufacturing practice (GMP)-compliant protocols. Here, the derivation and differentiation of functional dopaminergic neurons from hESCs and iPSCs under xeno-free defined conditions are described. These protocols have been validated in multiple hESC and iPSC lines.
人类胚胎干细胞（hESCs）和诱导多能干细胞（iPSCs）可能是细胞替代疗法和个性化医疗中无限的细胞来源。然而，在基于hESC和iPSC的疗法能够从实验室走向临床之前，必须建立在明确条件下生成治疗相关细胞的方案，比如在适合规模化、符合良好生产规范（GMP）的方案的特定条件下生成多巴胺能神经元。在此，我们描述了在无动物源明确条件下从hESCs和iPSCs中衍生并分化出功能性多巴胺能神经元的方法。这些方案已在多个hESC和iPSC系中得到验证。
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