Mirzoev T M, Biriukov N S, Veselova O M, Larina I M, Shenkman B S, Ogneva I V
Biofizika. 2012 May-Jun;57(3):509-14.
The aim of the work was to study the parameters of fibers cell respiration and desmin content in Wistar rat soleus muscle after 1, 3, 7 and 14 days of gravitational unloading. Gravitational unloading was simulated by antiorthostatic hindlimb suspension. The parameters of cell respiration were determined using the polarography, and desmin content was assessed by means of Western blotting. The results showed that the intensity of cell respiration is reduced after three days of gravitational unloading, reaches a minimum level after seven days and slightly increases by the fourteenth day of hindlimb unloading, as well as the content of desmin, which, however, to the fourteenth day returns to the control level. Taking into account that mitochondrial function depends on the state of cytoskeleton the data allow us to assume that early reduction of the intensity of cell respiration under unloading could be caused by degradation of the protein desmin that determines intracellular localization of mitochondria.
这项工作的目的是研究在重力卸载1、3、7和14天后，Wistar大鼠比目鱼肌中纤维细胞呼吸参数和结蛋白含量。通过抗直立性后肢悬吊模拟重力卸载。使用极谱法测定细胞呼吸参数，通过蛋白质免疫印迹法评估结蛋白含量。结果表明，重力卸载三天后细胞呼吸强度降低，七天后达到最低水平，后肢卸载第十四天时略有增加，结蛋白含量也是如此，不过到第十四天时恢复到对照水平。考虑到线粒体功能取决于细胞骨架的状态，这些数据使我们能够假设，卸载过程中细胞呼吸强度的早期降低可能是由决定线粒体细胞内定位的结蛋白降解所致。
