Yu Feng-xu, Zhang Ying, Tran Nuygen, Fu Yong, Liao Bin, Shi Ying-kang
Department of Cardiothoracic Surgery, Hospital Affiliated to Luzhou Medical College, Luzhou, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2012 May;40(5):392-6.
To observe the effect of direct myocardial injection of platelet rich plasma (PRP) on cardiac function, ventricular remodeling and myocardial perfusion.
Myocardial infarction was induced in 30 Fisher rats by left anterior descending coronary artery (LAD) ligation. One week after LAD ligation, rats in control group (n = 15) received 0.5 ml saline myocardial injection and rats in PRP group (n = 11) received 0.5 ml PRP myocardial injection.(99)Tc(m)-methoxyisobutylisonitrile (MIBI) gated single photon-emission computed tomography (SPECT) imaging was applied at 1 week post LAD ligation and prior myocardial saline/PRP injection and repeated at 4 week post LAD ligation to assess myocardial perfusion and ejection fraction (EF), left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV), and the number of infarct segments.
EF remained unchanged between 1 and 4 weeks post LAD ligation in control group and was significantly higher at 4 weeks post LAD ligation than at 1 week post LAD ligation in PRP group (P < 0.05). LVESV was significantly increased in the control group (P < 0.05) while remained unchanged in the PAP group (P < 0.05) at 4 weeks post LAD ligation compared that at 1 week post LAD ligation. LVEDV remained unchanged in the control group (P > 0.05) and significantly increased in the PRP group (P < 0.05) from 1 week to 4 weeks post LAD ligation. Myocardial perfusion remained unchanged in the control group and significantly improved in the PRP group at 4 week post LAD ligations. Histological examination also confirmed that PRP treatment can decrease infarct size [from (47 ± 19)% to (36 ± 11)%], increase ventricular wall thickness [from (3.1 ± 0.9) mm to(4.6 ± 1.8) mm] (P < 0.05).
Myocardial PRP injection could improve cardiac function and reperfusion in this rat model of acute myocardial infarction.
观察直接心肌注射富血小板血浆（PRP）对心脏功能、心室重构和心肌灌注的影响。
通过结扎左冠状动脉前降支（LAD）诱导30只Fisher大鼠发生心肌梗死。LAD结扎1周后，对照组（n = 15）大鼠接受0.5 ml生理盐水心肌注射，PRP组（n = 11）大鼠接受0.5 ml PRP心肌注射。在LAD结扎后1周且心肌注射生理盐水/PRP前进行（99）Tc（m）-甲氧基异丁基异腈（MIBI）门控单光子发射计算机断层扫描（SPECT）成像，并在LAD结扎后4周重复进行，以评估心肌灌注和射血分数（EF）、左心室舒张末期容积（LVEDV）、左心室收缩末期容积（LVESV）以及梗死节段数量。
对照组在LAD结扎后1至4周EF保持不变，而PRP组在LAD结扎后4周时EF显著高于LAD结扎后1周时（P < 0.05）。与LAD结扎后1周相比，LAD结扎后4周时对照组LVESV显著增加（P < 0.05），而PRP组LVESV保持不变（P < 0.05）。对照组LVEDV保持不变（P > 0.05），而PRP组在LAD结扎后1周至4周时LVEDV显著增加（P < 0.05）。对照组心肌灌注保持不变，而PRP组在LAD结扎后4周时心肌灌注显著改善。组织学检查也证实PRP治疗可减小梗死面积[从（47 ± 19）%降至（36 ± 11）%]，增加心室壁厚度[从（3.1 ± 0.9）mm增至（4.6 ± 1.8）mm]（P < 0.05）。
在该急性心肌梗死大鼠模型中，心肌注射PRP可改善心脏功能和再灌注。
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