Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
Nucleic Acids Res. 2012 Oct;40(19):9897-902. doi: 10.1093/nar/gks746. Epub 2012 Aug 9.
Systematic analysis of the RNA-protein interactome requires robust and scalable methods. We here show the combination of two completely orthogonal, generic techniques to identify RNA-protein interactions: PAR-CLIP reveals a collection of RNAs bound to a protein whereas SILAC-based RNA pull-downs identify a group of proteins bound to an RNA. We investigated binding sites for five different proteins (IGF2BP1-3, QKI and PUM2) exhibiting different binding patterns. We report near perfect agreement between the two approaches. Nevertheless, they are non-redundant, and ideally complement each other to map the RNA-protein interaction network.
系统分析 RNA-蛋白质相互作用组需要强大且可扩展的方法。我们在此展示了两种完全正交、通用的技术的结合，用于鉴定 RNA-蛋白质相互作用：PAR-CLIP 揭示了一组与蛋白质结合的 RNA，而基于 SILAC 的 RNA 下拉实验则鉴定了一组与 RNA 结合的蛋白质。我们研究了具有不同结合模式的五种不同蛋白质（IGF2BP1-3、QKI 和 PUM2）的结合位点。我们报告了这两种方法之间几乎完美的一致性。尽管如此，它们是非冗余的，理想情况下可以相互补充，以绘制 RNA-蛋白质相互作用网络。
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