亚零 24 小时非冻结大鼠心脏保存：一种新型可变磁场保存方法。
Subzero 24-hr nonfreezing rat heart preservation: a novel preservation method in a variable magnetic field.
机构信息
Department of General and Cardiothoracic Surgery, Kanazawa University, Kanazawa, Ishikawa, Japan.
出版信息
Transplantation. 2012 Sep 15;94(5):473-7. doi: 10.1097/TP.0b013e3182637054.
BACKGROUND
A new supercooling system using a variable magnetic field has been recently developed. Subzero nonfreezing preservation has been believed to be a beneficial method because of the lower metabolic rate. The purpose of this study was to evaluate the hemodynamic and metabolic effects of rat heart preservation in a variable magnetic field without cryoprotectants.
METHODS
Rat hearts were perfused ex vivo for 120 min after 24-hr preservation in two groups (n=6 each): (1) conventional storage group, in which the hearts were stored at 4°C, and (2) the subzero group, in which the hearts were preserved at -3°C in a variable magnetic field.
RESULTS
Reperfusion cardiac performance after preservation was significantly preserved in the subzero group compared with the conventional group with respect to heart rate, coronary flow, the peak positive dP/dt, and the peak negative dP/dt (P<0.05). Edema after reperfusion was significantly decreased (P<0.05), and the adenosine triphosphate level was higher in the subzero group (P<0.05).
CONCLUSIONS
The rat hearts preserved in a variable magnetic field at -3°C showed better hemodynamic and metabolic performance than those preserved using conventional storage at 4°C.
背景
最近开发了一种使用可变磁场的新型超冷却系统。由于代谢率较低，亚零非冻结保存被认为是一种有益的方法。本研究的目的是评估在没有冷冻保护剂的情况下使用可变磁场保存大鼠心脏的血液动力学和代谢效应。
方法
在两组（每组 n=6）中，大鼠心脏在 4°C 下储存 24 小时后进行离体灌注 120 分钟：（1）常规储存组，（2）亚零组，其中心脏在-3°C 的可变磁场中保存。
结果
与常规组相比，亚零组在再灌注后心脏性能得到了显著保存，表现在心率、冠脉流量、最大正 dp/dt 和最大负 dp/dt 方面（P<0.05）。再灌注后的水肿明显减少（P<0.05），亚零组的三磷酸腺苷水平更高（P<0.05）。
结论
在-3°C 的可变磁场中保存的大鼠心脏的血液动力学和代谢性能优于在 4°C 下使用常规保存的心脏。
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