用⁹⁹mTc对带有组氨酸标签的纳米抗体进行位点特异性标记：实用指南。
Site-specific labeling of his-tagged Nanobodies with ⁹⁹mTc: a practical guide.
作者信息
Xavier Catarina, Devoogdt Nick, Hernot Sophie, Vaneycken Ilse, D'Huyvetter Matthias, De Vos Jens, Massa Sam, Lahoutte Tony, Caveliers Vicky
机构信息
In Vivo Cellular and Molecular Imaging (ICMI) Laboratory, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
出版信息
Methods Mol Biol. 2012;911:485-90. doi: 10.1007/978-1-61779-968-6_30.
99mTc-tricarbonyl chemistry provides an elegant technology to site-specifically radiolabel histidine-tagged biomolecules. Considering their unique biochemical properties, this straightforward technology is particularly suited for Nanobodies. This chapter gives a detailed guide to generate highly specific Nanobody-derived radiotracers for both in vitro binding studies and in vivo molecular imaging.
99mTc-三羰基化学提供了一种用于位点特异性放射性标记组氨酸标签生物分子的精妙技术。鉴于其独特的生化特性，这种直接的技术特别适用于纳米抗体。本章详细介绍了如何生成用于体外结合研究和体内分子成像的高特异性纳米抗体衍生放射性示踪剂。
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