School of Cosmetic Science, Mae Fah Luang University, Chiang Rai, Thailand.
Pharm Biol. 2012 Nov;50(11):1384-90. doi: 10.3109/13880209.2012.675342. Epub 2012 Sep 11.
Litchi chinensis Sonn. (Spindaceae) is an important economic fruit of Thailand. Therapeutic effects of the fruits are contributed by anti-inflammatory phenolics.
To extract the litchi fruit pericarp in order to identify biologically actives substances with potential for cosmetic application.
The litchi pericarp was macerated by 70% ethanol (EtOH) and partitioned using n-hexane and ethyl acetate (EtOAc). In vitro antioxidant activities were assessed by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), ABTS and ferric reducing ability of plasma (FRAP) assays including tyrosinase inhibitory effect. Cellular radical scavenging capacity was monitored in a normal human fibroblast cell culture (NHF). Total phenolic content was determined and characterized by HPLC.
The EtOAc fraction was a significant antioxidant, stronger than ascorbic acid (p < 0.01), as assessed by ABTS (IC(50) = 7.137 ± 0.021 μg/mL), DPPH (IC(50) = 2.288 ± 0.063 μg/mL) and FRAP (EC(1mMFeSO4) = 8013.183 ± 58.804 μg/mL) assays. It demonstrated an antityrosinase effect (IC(50) = 197.860 ± 1.230 μg/mL) and showed no cytotoxic activity toward Vero and NHF cells, at a maximum tested concentration (50 μg/mL), with cellular antioxidant activity. Total phenolic content was highest in the most potent antioxidant fraction. Quercetin, rosmarinic and gallic acids were found. Total phenolic content is highly related to FRAP, antityrosinase, and ABTS activities.
Pericarp from litchi fruit can be obtained abundantly from agricultural waste, and the strong antioxidant activity demonstrated in this report may have application in topical cosmetic products. This ecological antioxidant can be prepared using a feasible method resulting in less waste and increased agro-industrial profitability.
荔枝（Litchi chinensis Sonn.）是泰国的一种重要经济水果。果实的治疗效果归因于具有抗炎作用的酚类物质。
提取荔枝果皮，以鉴定具有潜在化妆品应用价值的生物活性物质。
荔枝果皮用 70%乙醇（EtOH）浸渍，用正己烷和乙酸乙酯（EtOAc）萃取。通过 1,1-二苯基-2-苦基肼（DPPH）、ABTS 和血浆还原能力（FRAP）测定评估体外抗氧化活性，包括酪氨酸酶抑制作用。在正常人类成纤维细胞培养（NHF）中监测细胞自由基清除能力。用 HPLC 测定总酚含量并进行表征。
EtOAc 级分是一种有效的抗氧化剂，其抗氧化能力强于抗坏血酸（p<0.01），ABTS（IC50=7.137±0.021μg/mL）、DPPH（IC50=2.288±0.063μg/mL）和 FRAP（EC1mMFeSO4=8013.183±58.804μg/mL）测定均显示其具有抗氧化活性。它具有抗酪氨酸酶作用（IC50=197.860±1.230μg/mL），且在最大测试浓度（50μg/mL）下对 Vero 和 NHF 细胞无细胞毒性，具有细胞抗氧化活性。总酚含量在最有效的抗氧化级分中最高。发现了槲皮素、迷迭香酸和没食子酸。总酚含量与 FRAP、抗酪氨酸酶和 ABTS 活性高度相关。
荔枝果皮可从农业废弃物中大量获得，本报告中显示的强抗氧化活性可能在局部化妆品产品中有应用。这种生态抗氧化剂可以通过一种可行的方法制备，减少浪费，提高农业产业的盈利能力。
