Department of Thoracic Surgery, Kirikkale University, Kirikkale, Turkey.
Hum Exp Toxicol. 2012 Oct;31(10):1074-80. doi: 10.1177/0960327111428630. Epub 2012 Aug 14.
Lung cancer, which is mainly affected by environmental factors, is a lethal malignancy. It is also important to investigate the effect of genetic factors on lung cancer aetiology. In this study, we aimed to investigate the distribution of CYP1A1*2C, GSTT1 and GSTM1 polymorphisms in Turkish lung cancer patients to determine whether any promoting effect of polymorphisms could cause development of lung cancer. For this purpose, genomic DNA samples obtained from peripheral blood of 128 patients with lung cancer and 122 healthy subjects were analyzed. Genotyping of polymorphic enzymes were carried out by polymerase chain reaction-restriction fragment length polymorphism methods. Although there were no significant differences between groups in terms of CYP1A1 polymorphism, the carriers of CYP1A1 Ile/Val genotype (odds ratio [OR] = 1.224, 95% confidence interval [CI]: 0.585-2.564) or CYP1A1 Val/Val genotype (OR = 3.058, 95% CI: 0.312-30.303) had an increased risk of lung cancer development. There was no statistical difference between groups in terms of both GSTT1 null genotype (OR = 1.114, 95% CI: 0.590-2.105) and GSTM1 null genotype (OR = 0.776, 95% CI: 0.466-1.290). This is the first case-control study investigating CYP1A1 Ile/Val, GSTT1 and GSTM1 polymorphisms in Turkish lung cancer patients. Although we suggest that other genes in addition to the proposed genes could play a role in lung cancer development, the results of our study will contribute to the possible associations between CYP1A1 Ile/Val, GSTT1 and GSTM1 gene polymorphism on the risk of lung cancer.
肺癌主要受环境因素影响，是一种致命的恶性肿瘤。研究遗传因素对肺癌病因学的影响也很重要。在这项研究中，我们旨在调查 CYP1A1*2C、GSTT1 和 GSTM1 多态性在土耳其肺癌患者中的分布，以确定多态性的任何促进作用是否会导致肺癌的发展。为此，我们分析了来自 128 例肺癌患者和 122 例健康对照者外周血的基因组 DNA 样本。多态性酶的基因分型通过聚合酶链反应-限制性片段长度多态性方法进行。虽然 CYP1A1 多态性在组间无显著差异，但 CYP1A1 Ile/Val 基因型（比值比 [OR] = 1.224，95%置信区间 [CI]：0.585-2.564）或 CYP1A1 Val/Val 基因型（OR = 3.058，95% CI：0.312-30.303）的携带者患肺癌的风险增加。GSTT1 缺失基因型（OR = 1.114，95% CI：0.590-2.105）和 GSTM1 缺失基因型（OR = 0.776，95% CI：0.466-1.290）在组间无统计学差异。这是首例在土耳其肺癌患者中研究 CYP1A1 Ile/Val、GSTT1 和 GSTM1 多态性的病例对照研究。尽管我们认为除了提出的基因之外，其他基因可能在肺癌的发展中起作用，但我们的研究结果将有助于探讨 CYP1A1 Ile/Val、GSTT1 和 GSTM1 基因多态性与肺癌风险之间的可能关联。
