Johanning Karla, Hancock Gregg, Escher Beate, Adekola Adelbayo, Bernhard Mary Jo, Cowan-Ellsberry Christina, Domoradzki Jeanne, Dyer Scott, Eickhoff Curtis, Embry Michelle, Erhardt Susan, Fitzsimmons Patrick, Halder Marlies, Hill James, Holden Dustin, Johnson Rebecca, Rutishauser Sibylle, Segner Helmut, Schultz Irvin, Nichols John
KJohanning Consultancy, Pura Vida Connections LLC, Austin, Texas, USA.
Curr Protoc Toxicol. 2012 Aug;Chapter 14:Unit 14.10.1-28. doi: 10.1002/0471140856.tx1410s53.
Standard protocols are given for assessing metabolic stability in rainbow trout using the liver S9 fraction. These protocols describe the isolation of S9 fractions from trout livers, evaluation of metabolic stability using a substrate depletion approach, and expression of the result as in vivo intrinsic clearance. Additional guidance is provided on the care and handling of test animals, design and interpretation of preliminary studies, and development of analytical methods. Although initially developed to predict metabolism impacts on chemical accumulation by fish, these procedures can be used to support a broad range of scientific and risk assessment activities including evaluation of emerging chemical contaminants and improved interpretation of toxicity testing results. These protocols have been designed for rainbow trout and can be adapted to other species as long as species-specific considerations are modified accordingly (e.g., fish maintenance and incubation mixture temperature). Rainbow trout is a cold-water species. Protocols for other species (e.g., carp, a warm-water species) can be developed based on these procedures as long as the specific considerations are taken into account.
给出了使用肝脏S9组分评估虹鳟鱼代谢稳定性的标准方案。这些方案描述了从鳟鱼肝脏中分离S9组分、使用底物消耗方法评估代谢稳定性以及将结果表示为体内固有清除率。还提供了关于实验动物的护理和处理、初步研究的设计和解释以及分析方法开发的额外指导。尽管这些程序最初是为预测代谢对鱼类化学物质积累的影响而开发的，但它们可用于支持广泛的科学和风险评估活动，包括评估新兴化学污染物和改进毒性测试结果的解释。这些方案是针对虹鳟鱼设计的，只要相应地修改特定物种的考虑因素（例如，鱼类饲养和孵育混合物温度），就可适用于其他物种。虹鳟鱼是冷水物种。只要考虑到特定因素，就可以基于这些程序开发其他物种（例如，鲤鱼，一种温水物种）的方案。
