外周血乳腺珠蛋白基因表达用于乳腺癌患者转移的诊断和预测
Peripheral blood mammaglobin gene expression for diagnosis and prediction of metastasis in breast cancer patients.
作者信息
Radwan Wafaa M, Moussa Heba S, Essa Enas S, Kandil Samia H, Kamel Azza M
机构信息
Clinical Pathology Department, Faculty of Medicine, Menofiya University, Shebein El-Kom, Menofiya, Egypt.
出版信息
Asia Pac J Clin Oncol. 2013 Mar;9(1):66-70. doi: 10.1111/j.1743-7563.2012.01556.x. Epub 2012 Jul 4.
AIM
To evaluate the value of peripheral blood mammaglobin (MG) gene expression for diagnosis and prediction of metastasis in breast cancer patients.
METHODS
MG expression was detected by nested reverse-transcription polymerase chain reaction in the peripheral blood of 46 females (32 breast cancer, 12 benign breast lesions, 2 no breast abnormalities). In total 28 breast cancer patients were followed up through a period of 34 months for the development of metastasis.
RESULTS
MG expression was detected in 16/32 (50%) breast cancer patients but not in patients with benign lesions or healthy participants. Five patients had metastasis at diagnosis. During the 34 months of follow up, five more MG-positive patients showed metastatic lesions and none of the MG negative patients who were followed up developed metastasis.
CONCLUSION
The study suggests blood MG expression is a specific molecular marker for detection of occult mammary carcinoma cells of patients with operable breast cancer. It might be of value as a predictor of subsequent metastasis. Large-scale studies and longer follow-up periods are needed.
目的
评估外周血乳腺珠蛋白（MG）基因表达在乳腺癌患者诊断及转移预测中的价值。
方法
采用巢式逆转录聚合酶链反应检测46名女性（32例乳腺癌、12例乳腺良性病变、2例无乳腺异常）外周血中的MG表达。对28例乳腺癌患者进行了为期34个月的随访，观察转移情况。
结果
16/32（50%）的乳腺癌患者检测到MG表达，而良性病变患者或健康参与者未检测到。5例患者在诊断时已有转移。在34个月的随访期间，又有5例MG阳性患者出现转移灶，而随访的MG阴性患者均未发生转移。
结论
该研究表明，血液MG表达是可手术乳腺癌患者隐匿性乳腺癌细胞检测的特异性分子标志物。它可能作为后续转移的预测指标具有一定价值。需要进行大规模研究和更长时间的随访。
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