[LATS对小鼠甲状腺刺激的各种指标的比较（作者译）]

[A comparison of various indicators of murine thyroid stimulation by LATS (author's transl)].

作者信息

Eguchi N, Shimizu T, Shishiba Y

出版信息

Nihon Naibunpi Gakkai Zasshi. 1979 Oct 20;55(10):1307-12. doi: 10.1507/endocrine1927.55.10_1307.

DOI:10.1507/endocrine1927.55.10_1307

Abstract

To obtain an in vitro method of LATS assay which is equally sensitive to a McKenzie bioassay, we compared the quality of cAMP generation-stimulation, thyroidal adenyl cyclase stimulation, and the stimulation of T3-release from mouse thyroid slices. Among those indicators of thyroid stimulation, stimulation by IgG of the release of T3 from incubated thyroid slices is the most sensitive, reproducible and reliable indicator, reflecting faithfully the results of a McKenzie bioassay. Stimulation of cAMP generation in slice manifested less sensitivity, but reasonable reproducibility. However, adenyl cyclase stimulation by IgG of patients with Graves' disease was erratic in two successive experiments, probably due to a non-specific effect of IgG. When adenyl cyclase stimulation was adopted as a sole indicator of thyroid stimulation, the results must be interpreted cautiously.

摘要

为获得一种对麦肯齐生物测定法同样敏感的体外促甲状腺素（LATS）测定方法，我们比较了环磷酸腺苷（cAMP）生成刺激、甲状腺腺苷酸环化酶刺激以及从小鼠甲状腺切片中刺激三碘甲状腺原氨酸（T3）释放的质量。在那些甲状腺刺激指标中，孵育的甲状腺切片中IgG刺激T3释放是最敏感、可重复且可靠的指标，能如实地反映麦肯齐生物测定法的结果。切片中环磷酸腺苷生成的刺激表现出较低的敏感性，但有合理的可重复性。然而，格雷夫斯病患者的IgG对腺苷酸环化酶的刺激在连续两次实验中不稳定，可能是由于IgG的非特异性作用。当采用腺苷酸环化酶刺激作为甲状腺刺激的唯一指标时，对结果的解释必须谨慎。