Department of Biology of Aging, Section Stem Cell Biology, European Research Institute for the Biology of Aging, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
Ann N Y Acad Sci. 2012 Aug;1266:138-50. doi: 10.1111/j.1749-6632.2012.06549.x.
Attempts to improve hematopoietic reconstitution and engraftment potential of ex vivo-expanded hematopoietic stem and progenitor cells (HSPCs) have been largely unsuccessful due to the inability to generate sufficient stem cell numbers and to excessive differentiation of the starting cell population. Although hematopoietic stem cells (HSCs) will rapidly expand after in vivo transplantation, experience from in vitro studies indicates that control of HSPC self-renewal and differentiation in culture remains difficult. Protocols that are based on hematopoietic cytokines have failed to support reliable amplification of immature stem cells in culture, suggesting that additional factors are required. In recent years, several novel factors, including developmental factors and chemical compounds, have been reported to affect HSC self-renewal and improve ex vivo stem cell expansion protocols. Here, we highlight early expansion attempts and review recent development in the extrinsic control of HSPC fate in vitro.
由于无法产生足够数量的干细胞，并且起始细胞群体过度分化，体外扩增造血干细胞和祖细胞（HSPCs）的尝试在很大程度上都未能成功。尽管造血干细胞（HSCs）在体内移植后会迅速扩增，但体外研究经验表明，在培养中控制 HSPC 自我更新和分化仍然很困难。基于造血细胞因子的方案未能支持在培养中可靠地扩增未成熟的干细胞，这表明需要其他因素。近年来，已经有报道称包括发育因子和化学化合物在内的几种新型因子影响 HSC 自我更新并改进体外干细胞扩增方案。在这里，我们重点介绍早期的扩增尝试，并回顾体外 HSPC 命运的外在控制的最新进展。
