School of Life Sciences and State Kay Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, People's Republic of China.
PLoS One. 2012;7(8):e41822. doi: 10.1371/journal.pone.0041822. Epub 2012 Aug 8.
Glandular secreting trichomes (GSTs) are called biofactories because they are active in synthesizing, storing and secreting various types of plant secondary metabolites. As the most effective drug against malaria, artemisinin, a sesquiterpene lactone is derived from GSTs of Artemisia annua. However, low artemisinin content (0.001%~1.54% of dry weight) has hindered its wide application. We investigate the GST-expressed proteins in Artemisia annua using a comparative proteomics approach, aiming for a better understanding of the trichome proteome and arteminisin metabolism. 2D-electrophoresis was employed to compare the protein profiles of GSTs and leaves. More than 700 spots were resolved for GSTs, of which ∼93 non-redundant proteins were confidently identified by searching NCBI and Artemisia EST databases. Over 70% of these proteins were highly expressed in GTSs. Functional classification of these GSTs enriched proteins revealed that many of them participate in major plant metabolic processes such as electron transport, transcription and translation.
腺毛分泌型毛状体（GSTs）被称为生物工厂，因为它们在合成、储存和分泌各种类型的植物次生代谢物方面非常活跃。青蒿素是一种倍半萜内酯，作为对抗疟疾最有效的药物，它来源于黄花蒿 GSTs。然而，青蒿素含量低（干重的 0.001%~1.54%）限制了其广泛应用。我们采用比较蛋白质组学方法研究黄花蒿中的 GST 表达蛋白，旨在更好地了解毛状体蛋白质组和青蒿素代谢。二维电泳被用来比较 GST 和叶片的蛋白质图谱。GSTs 可分辨出 700 多个斑点，通过在 NCBI 和 Artemisia EST 数据库中搜索，可确定其中约 93 个非冗余蛋白。这些蛋白中有 70%以上在 GSTs 中高度表达。对这些 GSTs 富集蛋白的功能分类表明，它们中的许多蛋白参与主要的植物代谢过程，如电子传递、转录和翻译。
