Department of Surgery, Michigan State University, Lansing, MI 48912, USA.
Am J Surg. 2012 Nov;204(5):598-601. doi: 10.1016/j.amjsurg.2012.07.004. Epub 2012 Aug 17.
Understanding gut development may illuminate the adaptive response to massive small-bowel resection and facilitate enteral nutrition. We reported that Schlafen-3 (Slfn3) mediates differentiation in vitro in rat intestinal epithelial. We hypothesized that Slfn3 is involved in intestinal development in vivo.
We removed fetal intestines, liver, and lungs on day 20 of gestation, at birth, and on postnatal days 1 and 5. Expression of Slfn3, markers of intestinal differentiation, and Slfn5, to address specificity, were determined by quantitative reverse-transcription polymerase chain reaction.
Villin expression increased on days 1 and 5 (8.7 ± .6 and 5.4 ± .4, respectively; P < .01). Intestinal Slfn3 expression was increased substantially after birth (2.1- ± .5-fold) and on days 1 and 5 (P < .02). Slfn3 was higher after birth in liver and lung but decreased sharply thereafter. Slfn5 expression was mostly unchanged.
The data suggest that the developmental/maturation effects we observed correlate with Slfn3 but not Slfn5 and are more relevant to the intestines. A better understanding of how Slfn3 promotes intestinal differentiation could help promote intestinal maturation, improving outcomes in children or adults with short-gut syndrome.
了解肠道发育可以阐明对大量小肠切除的适应性反应，并有助于肠内营养。我们报道 Schlafen-3（Slfn3）在大鼠肠上皮细胞的体外分化中起介导作用。我们假设 Slfn3 参与体内肠道发育。
我们在妊娠第 20 天、出生时以及出生后第 1 天和第 5 天取出胎儿的肠、肝和肺。通过定量逆转录聚合酶链反应确定 Slfn3 的表达、肠分化标志物和 Slfn5 的表达，以解决特异性问题。
绒毛蛋白表达在第 1 天和第 5 天增加（分别为 8.7±0.6 和 5.4±0.4；P<.01）。肠 Slfn3 表达在出生后显著增加（2.1-0.5 倍），在第 1 天和第 5 天增加（P<.02）。出生后 Slfn3 在肝和肺中的表达较高，但此后急剧下降。Slfn5 表达基本不变。
数据表明，我们观察到的发育/成熟效应与 Slfn3 相关，但与 Slfn5 无关，与肠道的相关性更强。更好地了解 Slfn3 如何促进肠分化可能有助于促进肠成熟，改善短肠综合征儿童或成人的预后。
