通过流式细胞术从足月羊膜绒毛膜中纯化人细胞滋养层细胞。

Purification of human cytotrophoblast from term amniochorion by flow cytometry.

作者信息

Shorter S C, Jackson M C, Sargent I L, Redman C W, Starkey P M

机构信息

Harris-Birthright Pre-eclampsia Unit, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Maternity Department, Headington, Oxford, UK.

出版信息

Placenta. 1990 Nov-Dec;11(6):505-13. doi: 10.1016/s0143-4004(05)80196-1.

DOI:10.1016/s0143-4004(05)80196-1

PMID:2290802

Abstract

Term cytotrophoblast do not express polymorphic MHC Class I antigens, unlike other fetal and maternal cells in the amniochorion/decidua. This allows cytotrophoblast to be isolated and purified from this tissue, utilizing 4E, a monoclonal antibody specific for HLA-B, which labels only non-trophoblast. We have developed a method using enzymic dispersion and Percoll gradient centrifugation, followed by flow cytometry, that yields, on average, a total of 5 X 10(6) term extravillous cytotrophoblast, 97 per cent pure. The availability of highly purified extravillous cytotrophoblast, for the first time, permits precise investigation of trophoblast function.

摘要

与羊膜绒毛膜/蜕膜中的其他胎儿和母体细胞不同，细胞滋养层不表达多态性MHC I类抗原。这使得细胞滋养层能够从该组织中分离和纯化出来，利用4E（一种对HLA - B特异的单克隆抗体，它只标记非滋养层细胞）。我们开发了一种方法，即先用酶分散和Percoll梯度离心，然后进行流式细胞术，平均可产生总共5×10⁶个足月绒毛外细胞滋养层，纯度为97%。首次获得高度纯化的绒毛外细胞滋养层，使得能够精确研究滋养层功能。