INRES - Chemical Signalling, University of Bonn, Friedrich-Ebert-Allee 144, 53113 Bonn, Germany.
Trends Cell Biol. 2012 Oct;22(10):503-8. doi: 10.1016/j.tcb.2012.07.007. Epub 2012 Aug 20.
Mitochondrial free radicals and redox poise are central to metabolism and cell fate. Their measurement in living cells remains a major challenge and their in vivo dynamics are poorly understood. Reports of 'superoxide flashes' in single mitochondria have therefore been perceived as a major breakthrough: single mitochondria expressing the genetically encoded sensor circularly permuted yellow fluorescent protein (cpYFP) display spontaneous flashes of fluorescence that are responsive to metabolic changes and stressors. We critically review the evidence that underpins the interpretation of mitochondrial cpYFP flashes as bursts of superoxide production and conclude that flashes do not represent superoxide bursts but instead are caused by transient alkalinisation of the mitochondrial matrix. We provide a revised framework that will help to clarify the interpretation of mitochondrial flashes.
线粒体自由基和氧化还原平衡是新陈代谢和细胞命运的核心。在活细胞中测量它们仍然是一个主要挑战，它们的体内动力学还知之甚少。因此，关于单个线粒体中“超氧化物闪光”的报道被认为是一个重大突破：表达遗传编码传感器环状排列黄色荧光蛋白 (cpYFP) 的单个线粒体显示出对代谢变化和应激源有反应的自发荧光闪烁。我们批判性地审查了支持将线粒体 cpYFP 闪光解释为超氧化物产生爆发的证据，并得出结论，闪光并不代表超氧化物爆发，而是由线粒体基质的瞬时碱化引起的。我们提供了一个修订后的框架，这将有助于澄清线粒体闪光的解释。
