Biology Department, Faculty of Science, Taif University (Qurwa), Taif, Saudi Arabia.
Ecotoxicol Environ Saf. 2012 Oct;84:347-54. doi: 10.1016/j.ecoenv.2012.08.014. Epub 2012 Aug 22.
The present study aimed to investigate the efficacy of antioxidant defense system of the land snail Eobania vermiculata as biomarkers for terrestrial heavy metal pollution. For this reason, a set of biomarkers was performed on land snails E. vermiculata collected from polluted and non-polluted areas in the field. The biomarkers used were lactate dehydrogenase activity, level of lipid peroxidation, activities of catalase, glutathione peroxidase, glutathione-S-transferase, gamma-glutamyl transferase and content of glutathione, metalothionines, total proteins and total lipid in the digestive gland tissue. The correlation between the bioaccumulation of heavy metal in gland tissue and all the biomarkers in the digestive gland was determined. The results revealed appreciable alterations in the biomarker values in field, accompanied by significant correlations among the biomarkers. Therefore, this study suggests E. vermiculata is a suitable sentinel organism and the selected biomarkers show efficacy for terrestrial heavy metal biomonitoring.
本研究旨在探讨陆生蜗牛 Eobania vermiculata 抗氧化防御系统作为陆地重金属污染生物标志物的功效。为此，我们在野外采集了来自污染和未污染地区的陆生蜗牛 E. vermiculata ，并对其进行了一系列生物标志物检测。所用的生物标志物包括乳酸脱氢酶活性、脂质过氧化水平、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽-S-转移酶、γ-谷氨酰转移酶和谷胱甘肽含量、金属硫蛋白、总蛋白和总脂质在消化腺组织中的含量。确定了重金属在组织中的生物积累与消化腺中所有生物标志物之间的相关性。结果表明，生物标志物值在野外发生了明显变化，并且生物标志物之间存在显著相关性。因此，本研究表明 E. vermiculata 是一种合适的指示生物，所选生物标志物对陆地重金属生物监测具有有效性。
