Department of Clinical Laboratory, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, PR China.
Mol Med Rep. 2012 Nov;6(5):1069-74. doi: 10.3892/mmr.2012.1035. Epub 2012 Aug 16.
The aim of this study was to investigate the relationship between E-selectin +G98T, +A561C polymorphisms and the levels of hepatitis B virus (HBV) DNA and preS1 antigen (preS1Ag) in patients with chronic hepatitis B (CHB) infection. Polymorphisms of the E-selectin gene in 150 CHB patients and 150 healthy controls of two different nationalities were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Real-time quantitative PCR was used to detect the levels of HBV DNA. preS1Ag and five items of hepatitis B were detected by enzyme-linked immunosorbent assay. Two genotypes, GG (94%, 96%) and GT (6%, 4%) of the E-selectin +G98T polymorphism, and AA (78.67%, 80.67%) and AC (21.33%, 19.33%) of the +A561C polymorphism, were found in these patients. There were also significant differences in the two nationalities in the genotypic frequencies in +A561C polymorphisms between patients and healthy subjects (χ2=5.489, χ2=5.653; P<0.05). In the patients studied, the relative risk of suffering from CHB in genotype AC was 2.122 and 2.313-fold higher for the two nationalities, respectively, than that of the AA genotype (OR=2.122, 95% CI 1.121-4.019; OR=2.313, 95% CI 1.002-5.360). There was also significant over-representation in the C allele frequency between the two groups (χ2=5.000, χ2=5.30; P<0.05), and the levels of HBV DNA and preS1Ag in the AC genotype patients were higher than those in the AA genotype (P<0.01 and P<0.05). E-selectin +A561C and +G98T polymorphisms were present in the populations studied. Therefore, there is a correlation between E-selectin +A561C polymorphisms and CHB. Allele C may be one of the predisposing factors, and mutation of this locus may impact the virus copy number.
本研究旨在探讨 E-选择素+G98T、+A561C 多态性与慢性乙型肝炎（CHB）患者乙型肝炎病毒（HBV）DNA 及前 S1 抗原（preS1Ag）水平之间的关系。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测 150 例 CHB 患者和 150 例不同国籍健康对照者 E-选择素基因多态性，实时定量 PCR 检测 HBV DNA 水平，酶联免疫吸附试验检测 preS1Ag 及乙肝五项。发现患者 E-选择素+G98T 多态性存在 GG（94%，96%）和 GT（6%，4%）两种基因型，+A561C 多态性存在 AA（78.67%，80.67%）和 AC（21.33%，19.33%）两种基因型，且两种多态性在患者和健康对照者的基因型频率在不同国籍之间存在显著差异（χ2=5.489，χ2=5.653；P<0.05）。在研究患者中，与 AA 基因型相比，AC 基因型在两个国籍中的 CHB 发病风险分别高 2.122 和 2.313 倍（OR=2.122，95%CI 1.121-4.019；OR=2.313，95%CI 1.002-5.360）。两组间 C 等位基因频率也明显偏高（χ2=5.000，χ2=5.30；P<0.05），AC 基因型患者的 HBV DNA 和 preS1Ag 水平高于 AA 基因型患者（P<0.01 和 P<0.05）。本研究人群存在 E-选择素+A561C 和+G98T 多态性。因此，E-选择素+A561C 多态性与 CHB 之间存在相关性。等位基因 C 可能是一个易感因素，该位点突变可能影响病毒载量。
