Iyer R R, Prasad H K, Bhutani L K, Rao D N
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
Int J Immunopharmacol. 1990;12(8):859-69. doi: 10.1016/0192-0561(90)90005-8.
The ability of blood monocyte/macrophages from normal donors, tuberculoid leprosy (BT/TT) and lepromatous leprosy (BL/LL) patients to exert enhanced microbicidal activity was assayed after stimulating with 0.8 microM tuftsin, as a function of the duration of cultures in vitro. Normal and BT/TT macrophage cultures showed a statistically significant increase in microbicidal activity against Staphylococcus aureus at all ages of culture (6 h to 14 days), though the overall magnitude of the enhancement shows a decrease with increasing culture age in the same populations. However, 14-day old BL/LL macrophage cultures were unable to undergo tuftsin-mediated stimulation of microbicidal activity against S. aureus and even, fresh 6 h-old cultures exhibited a tuftsin-stimulated response profile similar to 14-day old normal and BT/TT cultures. Also, 7 and 14-day cultures of normal, BT/TT and BL/LL macrophages were unable to inhibit/kill intracellular Mycobacterium leprae after a single stimulation with 0.8 microM tuftsin. However, serial, daily stimulation with 0.8 microM tuftsin resulted in 77-140% inhibition of 3H-thymidine uptake by the 12th day of cultures in vitro in the three groups. These results suggest that BL/LL macrophages exhibit a premature inability to undergo tuftsin stimulated microbicidal activity, which may possibly be reversed by serial dosage of tuftsin.
用0.8微摩尔的促吞噬肽刺激后，检测了正常供体、结核样麻风（BT/TT）和瘤型麻风（BL/LL）患者血液中的单核细胞/巨噬细胞发挥增强杀菌活性的能力，这是体外培养持续时间的函数。正常和BT/TT巨噬细胞培养物在所有培养阶段（6小时至14天）对金黄色葡萄球菌的杀菌活性均有统计学显著增加，尽管在同一群体中，随着培养时间的增加，增强的总体幅度有所下降。然而，14天龄的BL/LL巨噬细胞培养物无法接受促吞噬肽介导的对金黄色葡萄球菌杀菌活性的刺激，甚至新鲜的6小时龄培养物表现出的促吞噬肽刺激反应模式与14天龄的正常和BT/TT培养物相似。此外，正常、BT/TT和BL/LL巨噬细胞的7天和14天培养物在用0.8微摩尔促吞噬肽单次刺激后，无法抑制/杀死细胞内麻风杆菌。然而，在体外培养的第12天，三组中用0.8微摩尔促吞噬肽连续每日刺激导致3H-胸腺嘧啶核苷摄取抑制77%-140%。这些结果表明，BL/LL巨噬细胞过早地表现出无法接受促吞噬肽刺激的杀菌活性，这可能通过促吞噬肽的连续给药而逆转。
