Han H, Takase S, Nishino N
Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
Lett Appl Microbiol. 2012 Nov;55(5):384-9. doi: 10.1111/j.1472-765X.2012.03305.x. Epub 2012 Sep 11.
To determine the survival rate of silage lactic acid bacteria (LAB) in the ruminant gastrointestinal tract.
Wilted Italian ryegrass (Lolium multiflorum Lam.) silage (containing 1·9×10(6) CFU LAB g(-1)) was fed ad libitum to three goats equipped with rumen cannulae. Silage was given alone or with concentrates at a 1:1 ratio on a dry matter basis. Rumen fluid was then obtained 2, 4 and 8h after the morning feeding. Denaturing gradient gel electrophoresis was performed to compare LAB communities in silage, rumen fluid and faeces. The LAB detected in the wilted silage included Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus murinus and Lactobacillus sakei. Bands indicative of Lact. murinus were detected in either the rumen fluid or faeces, whereas the bands indicative of Lact. plantarum, Lact. brevis and Lact. sakei were not. Although the rumen fluid LAB counts and volatile fatty acid concentrations were higher in goats fed silage plus concentrates compared with those fed silage alone, the LAB communities themselves remained unaffected. Sampling times and goat-to-goat variations did not affect the LAB communities found in the rumen fluid.
LAB communities found in the gut are not remarkably affected by the consumption of silage LAB, even when the silage is accompanied by concentrates that facilitate gut fermentation.
Although silage can improve probiotic function, it may be difficult for silage LAB to survive the digestive process in the ruminant gastrointestinal tract.
测定青贮饲料乳酸菌（LAB）在反刍动物胃肠道中的存活率。
将萎蔫的意大利黑麦草（多花黑麦草）青贮饲料（含有1·9×10⁶CFU LAB g⁻¹）随意喂给三只装有瘤胃瘘管的山羊。青贮饲料单独投喂或以干物质计1:1的比例与精饲料一起投喂。在早晨喂食后2、4和8小时采集瘤胃液。进行变性梯度凝胶电泳以比较青贮饲料、瘤胃液和粪便中的LAB群落。在萎蔫青贮饲料中检测到的LAB包括植物乳杆菌、短乳杆菌、鼠李糖乳杆菌和清酒乳杆菌。在瘤胃液或粪便中检测到了指示鼠李糖乳杆菌的条带，而指示植物乳杆菌、短乳杆菌和清酒乳杆菌的条带未被检测到。尽管与仅喂青贮饲料的山羊相比，喂青贮饲料加精饲料的山羊瘤胃液LAB计数和挥发性脂肪酸浓度更高，但LAB群落本身并未受到影响。采样时间和山羊个体差异并未影响瘤胃液中发现的LAB群落。
即使青贮饲料伴有促进肠道发酵的精饲料，肠道中发现的LAB群落也不会受到青贮饲料LAB消耗的显著影响。
尽管青贮饲料可以改善益生菌功能，但青贮饲料LAB可能难以在反刍动物胃肠道的消化过程中存活。
