Further study on gas phase acid hydrolysis of protein: improvement of recoveries for tryptophan, tyrosine, and methionine.
作者信息
Yano H, Aso K, Tsugita A
机构信息
Research Institute for Biosciences, Science University of Tokyo, Chiba.
出版信息
J Biochem. 1990 Oct;108(4):579-82. doi: 10.1093/oxfordjournals.jbchem.a123245.
Abstract
A novel method for vapor phase acid hydrolysis of protein suitable for quantitative analysis of tryptophan is presented. The hydrolysis is carried out in vapor of a mixture made of 7 M HCl, 10% trifluoroacetic acid, and 20% thioglycolic acid in the presence of indole. Reasonably good recoveries of common amino acids, including tryptophan (above 75%), were achieved.
摘要
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