Sun Dong-Yun, Sun Li-Hong, Liang Yu-Lei, Zhang Shu-Yi, Xiao Hong-Ling, Wu Zhong-Qiu, Li Wen-Li, Li Xin-Hua, Ma Hong-Li
Department of Acu-moxibustion, College of Chinese Medicine, Hebei Medical University, Shijiazhuang 050091 , China.
Zhen Ci Yan Jiu. 2012 Jun;37(3):206-10.
To investigate the influence of electroacupuncture (EA) on lipid metabolism in both sexes of obesity model rats.
A total of 30 male and 30 female SD rats were randomized into normal control, model and EA groups respectively, with 10 rats in each group. EA (2 Hz, 1-2 mA) was applied to the ipsilateral "Zusanli" (ST 36)-"Sanyin-jiao"(SP 6) and bilateral "Fenglong" (ST 40) for 30 min, once daily for 28 days. In addition,"Quchi" (LI 11),"Zhongwan" (CV 12) and "Guanyuan" (CV 4) were punctured with filiform needles and stimulated by manipulating the needle for 1 min, separately. Obesity model was duplicated by subcutaneous injection of 15% glutamic sodium (0.2 mL/10 g) once daily for 5 days and by feeding the animal with high fat forage for 36 days. Lee's index was calculated, and plasma triglyeride (TG) concentration detected by glycerol-phosphoric acid oxidase-peroxydase method, total cholesterol (TC) detected by cholesterol oxidase method, and plasma low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein-cholesterol (HDL-C) contents were assayed by one-step method, respectively.
In both male and female rats, compared to their own normal groups, the Lee's index, plasma TG, TC and LDL-C contents were increased significantly and plasma HDL-C contents decreased obviously in the model groups (P<0.01), while compared to their own model groups, the Lee's index, plasma TG, TC and LDL-C levels in the EA groups were down-regulated considerably and plasma HDL-C contents up-regulated remarkably after the treatment (P<0.01, P<0.05). The efficacies of the EA intervention were obviously better in reducing Lee's index for male rats, and in down-regulating plasma TG and TC contents in female rats (P<0.05). No significant differences were found between male and female rats in EA intervention for plasma LDL-C and HDL-C levels.
EA intervention can effectively regulate obesity rats' Lee's index and plasma TG, TC, LDL-C and HDL-C levels. The effects are different in male and female rats.
探讨电针（EA）对肥胖模型大鼠雌雄两性脂质代谢的影响。
将30只雄性和30只雌性SD大鼠分别随机分为正常对照组、模型组和电针组，每组10只。电针（2Hz，1 - 2mA）刺激同侧“足三里”（ST36）-“三阴交”（SP6）及双侧“丰隆”（ST40），每次30分钟，每日1次，共28天。此外，用毫针针刺“曲池”（LI11）、“中脘”（CV12）和“关元”（CV4），分别行提插捻转手法刺激1分钟。通过每日皮下注射15%谷氨酸钠（0.2mL/10g），连续5天，并给予高脂饲料喂养36天复制肥胖模型。计算Lee氏指数，采用甘油磷酸氧化酶 - 过氧化物酶法检测血浆甘油三酯（TG）浓度，胆固醇氧化酶法检测总胆固醇（TC），一步法分别检测血浆低密度脂蛋白胆固醇（LDL - C）和高密度脂蛋白胆固醇（HDL - C）含量。
在雄性和雌性大鼠中，与各自正常组相比，模型组的Lee氏指数、血浆TG、TC和LDL - C含量显著升高，血浆HDL - C含量明显降低（P<0.01）；而与各自模型组相比，电针组治疗后Lee氏指数、血浆TG、TC和LDL - C水平显著下调，血浆HDL - C含量显著上调（P<0.01，P<0.05）。电针干预在降低雄性大鼠Lee氏指数以及下调雌性大鼠血浆TG和TC含量方面效果明显更好（P<0.05）。电针干预对血浆LDL - C和HDL - C水平在雄性和雌性大鼠之间未发现显著差异。
电针干预可有效调节肥胖大鼠的Lee氏指数及血浆TG、TC、LDL - C和HDL - C水平。在雄性和雌性大鼠中的效果有所不同。
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