两种修饰核苷的缺氧选择性 DNA 链间交联形成。
Hypoxia-selective DNA interstrand cross-link formation by two modified nucleosides.
机构信息
Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee, 53211, USA.
出版信息
Chemistry. 2012 Oct 1;18(40):12609-13. doi: 10.1002/chem.201201960. Epub 2012 Aug 31.
Abstract
The clean crossed code: Two nitroimidazole-modified thymidines 1 a and 1 b were synthesized and incorporated into DNA oligomers. The 350 nm photolysis of 1 a and 1 b generated a 5-(2'-deoxyuridinyl)methyl radical that induced DNA interstrand cross-links (ICL; see scheme). A higher ICL yield was observed under hypoxic conditions than under aerobic conditions.
摘要
清洁交叉码
合成了两个硝基咪唑修饰的胸腺嘧啶 1a 和 1b，并将其掺入 DNA 寡核苷酸中。1a 和 1b 的 350nm 光解生成了 5-(2'-脱氧尿嘧啶基)甲基自由基，诱导 DNA 链间交联（ICL；见方案）。在缺氧条件下观察到比有氧条件下更高的 ICL 产率。
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