State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Bioresour Technol. 2012 Nov;123:92-7. doi: 10.1016/j.biortech.2012.07.093. Epub 2012 Aug 2.
The objective of the study was to test the hypothesis that the attachment of polycyclic aromatic hydrocarbons (PAHs)-degrading bacteria can promote desorption of PAHs from humin, thereby increasing their bioavailability. Biodegradation of humin-bound phenanthrene (PHE) - a model compound for PAHs - was investigated using two PHE-degrading bacteria, Sphingobium sp. PHE3 and Micrococcus sp. PHE9, respectively. Sorption data of PHE to humin fitted well into the modified Freundlich equation. Further, a new sorption band appeared at 1262cm(-1), demonstrating intermolecular interactions between PHE and humin. Interestingly, approximately 65.3% of humin-bound PHE was degraded by both strains, although only about 17.8% of PHE could be desorbed from humin by Tenax extraction. Furthermore, both strains grew well in mineral medium and also attached to humin surfaces for substrate uptake. It is proposed that the attached bacteria could possibly consume PHE on the humin via interactions between bacterial surfaces and humin, thereby overcoming the low PHE bioavailability and resulting in enhanced degradation.
本研究旨在验证以下假设，即多环芳烃（PAHs）降解菌的附着可以促进腐殖质中 PAHs 的解吸，从而增加其生物利用度。使用两种 PAHs 降解菌，即 Sphingobium sp. PHE3 和 Micrococcus sp. PHE9，分别研究了腐殖质结合芘（PHE）的生物降解情况-一种 PAHs 的模型化合物。PHE 对腐殖质的吸附数据很好地符合修正的 Freundlich 方程。此外，在 1262cm(-1)处出现了一个新的吸附带，表明 PHE 与腐殖质之间存在分子间相互作用。有趣的是，尽管 Tenax 萃取只能从腐殖质中解吸约 17.8%的 PHE，但两种菌株都能降解约 65.3%的腐殖质结合 PHE。此外，两种菌株在矿物培养基中生长良好，并且也附着在腐殖质表面以吸收底物。据推测，附着的细菌可能通过细菌表面与腐殖质之间的相互作用来消耗腐殖质上的 PHE，从而克服 PHE 生物利用度低的问题，并导致增强的降解。
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