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应用巢式 PCR 技术,从组织中提取 DNA 对 1 例返回中国的盘尾丝虫病患者进行诊断

A Case of loiasis in a patient returning to China diagnosed by nested PCR using DNA extracted from tissue.

机构信息

Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China.

出版信息

J Travel Med. 2012 Sep-Oct;19(5):314-6. doi: 10.1111/j.1708-8305.2012.00635.x. Epub 2012 Jul 30.

Abstract

Recognition and diagnosis of loiasis is problematic in areas where this infection is not endemic but may be imported. A previously healthy Chinese male returned from Equatorial Guinea presenting with migratory masses. He was diagnosed with loiasis following detection of Loa loa by nested polymerase chain reaction using DNA extracted from tissue.

摘要

在并非地方性流行而可能输入性传播的地区,罗阿丝虫病的识别和诊断存在问题。一名既往健康的中国男性从赤道几内亚返回,出现游走性肿块。使用从组织中提取的 DNA 通过巢式聚合酶链反应检测到罗阿丝虫后,诊断为罗阿丝虫病。

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