[Experimental study of constructing vertebral canal on rabbit model with bone marrow mesenchymal stem cells].

OBJECTIVE

To construct the artificial lamina of vertebral arch with bone marrow mesenchymal stem cells transplanted in collagen sponge on a rabbit model and observe the growth of new bone.

METHODS

To draw out the bone marrow blood from the femur of 2 weeks old rabbit and get the bone marrow mesenchymal stem cells by centrifugal and adhesive effect. To induce the MSCs to osteoblasts and transplant the induced cells in collagen sponge to construct the tissue engineering bone. To divide 48 rabbits into 3 groups randomly, namely group A, group B and group C. All of the rabbits are taken laminectomy in L6, and to group B and C, collagen sponge and tissue engineering bone are implanted in the operation area respectively. The artificial lamina of vertebral arch is determined qualitatively and quantitatively by methods including imageology and histomorphometry.

RESULTS

The artificial lamina of vertebral arch is successfully constructed 4 weeks after operation in group C, CT examination at 4 weeks shows that new lamina of vertebral arch is formed, and the vertebral canal is intact.

CONCLUSIONS

The artificial lamina of vertebral arch can be constructed successfully with the usage of tissue engineering bone transplanted bone marrow mesenchymal stem cells.