Fernandez E, Pallini R, Mercanti D
Institute of Neurosurgery, Catholic University, Rome, Italy.
Neurosurgery. 1990 Jan;26(1):37-42. doi: 10.1097/00006123-199001000-00005.
The effect of exogenous nerve growth factor (NGF) on axonal regeneration into autologous peripheral nerve (PN) grafts implanted to the spinal cord (SC) of rats was assessed by retrograde labeling of the parent soma of the regenerating axons with horseradish peroxidase. NGF was delivered at the graft site over periods of 15 and 30 days by using indwelling osmotic minipumps. In control rats, the minipumps were filled with saline. At 15 days after grafting in the NGF-treated rats, the mean number of SC as well as dorsal root ganglion (DRG) neurons that regenerated their axons into the peripheral nerve grafts was increased 55.3 and 26.4 times, respectively, as compared to the control group values. At 30 days, SC and DRG neurons in the NGF-treated group were 10.9 and 3.1 times greater than in the control group. In the NGF-treated group, the regenerating SC neurons were located within a range of 7 to 13 mm from the graft site as compared to 1 to 7 mm in the control group. Finally, the analysis of the soma diameters of the regenerating neurons showed that NGF enhanced and maintained with time the regenerative response from small-sized DRG neurons. Therefore, NGF is thought to promote directly the regenerative potential of SC as well as DRG neurons and to exert an indirect glial cell-mediated effect at the SC-graft interface.
通过用辣根过氧化物酶对再生轴突的亲代胞体进行逆行标记，评估外源性神经生长因子（NGF）对植入大鼠脊髓（SC）的自体周围神经（PN）移植物中轴突再生的影响。使用留置渗透微型泵在移植物部位给予NGF 15天和30天。在对照大鼠中，微型泵中填充生理盐水。在NGF处理的大鼠移植后15天，与对照组相比，其轴突再生至周围神经移植物中的脊髓以及背根神经节（DRG）神经元的平均数量分别增加了55.3倍和26.4倍。在30天时，NGF处理组中的脊髓和DRG神经元比对照组分别多10.9倍和3.1倍。在NGF处理组中，再生的脊髓神经元位于距移植物部位7至13毫米的范围内，而对照组为1至7毫米。最后，对再生神经元胞体直径的分析表明，NGF增强并随时间维持了小型DRG神经元的再生反应。因此，人们认为NGF直接促进脊髓以及DRG神经元的再生潜力，并在脊髓-移植物界面发挥间接的胶质细胞介导作用。
