西曲瑞克和曲普瑞林对人黄素化颗粒细胞培养活力和类固醇生成的影响。

The effects of cetrorelix and triptorelin on the viability and steroidogenesis of cultured human granulosa luteinized cells.

机构信息

Laboratory of Physiology, School of Medicine, Democritus University of Thrace, Dragana, 68100 Alexandroupolis, Greece.

出版信息

In Vivo. 2012 Sep-Oct;26(5):835-9.

Abstract

BACKGROUND

We investigated the effects of the gonadotropin-releasing hormone (GnRH) agonist triptorelin as well the GnRH antagonist cetrorelix those of on the viability and steroidogenesis in human granulosa luteinized (hGL) cell cultures.

MATERIALS AND METHODS

The hGL cells were obtained from 34 women undergoing ovarian stimulation for IVF treatment. The cells were cultured for 48 h with or without 1 nM or 3 nM of cetrorelix or triptorelin in serum-free media. The cell viability was evaluated by the MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay. The concentrations of estradiol and progesterone in culture supernatants were measured by ELISA.

RESULTS

Treatment with triptorelin slightly increased cell viability, whereas treatment with 3 nM cetrorelix led to a significant decrease. Estradiol concentrations were reduced with 3 nM triptorelin. Cultures treated with high-dose of either cetrorelix or triptorelin tended to secrete less progesterone than controls.

CONCLUSION

Cetrorelix significantly reduces the viability of hGL cells. Triptorelin and cetrorelix may have minor effects on steroidogenesis. These results suggest that GnRH analogues may influence ovarian functions.

摘要

背景

我们研究了促性腺激素释放激素（GnRH）激动剂曲普瑞林和 GnRH 拮抗剂西曲瑞克对人卵巢黄素化颗粒细胞（hGL）培养物中的细胞活力和类固醇生成的影响。

材料和方法

hGL 细胞取自 34 名接受 IVF 治疗的卵巢刺激的女性。将细胞在无血清培养基中与 1 nM 或 3 nM 的西曲瑞克或曲普瑞林孵育 48 小时。通过 MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴化物]测定评估细胞活力。通过 ELISA 测定培养上清液中雌二醇和孕酮的浓度。