Department of Dermatology, The Third People's Hospital of Hangzhou, Hangzhou, Zhejiang, China.
Mycoses. 2013 Mar;56(2):130-3. doi: 10.1111/j.1439-0507.2012.02222.x. Epub 2012 Sep 10.
Traditional diagnostic testing for dermatophyte infection currently requires skin scraping for light microscopy and/or fungal culture or skin biopsy. Immunofluorescent microscopy can also be used with calcofluor stain. All of these tests can be time-consuming to perform, require a waiting period for results and are invasive. This study aimed to define the in vivo reflectance confocal microscopy (RCM) features of superficial cutaneous fungal infections and to analyse concordance with microscopic examination. Totally, 45 patients, who were diagnosed with superficial cutaneous fungal infections according to the positive result of microscopic examination, were enrolled in this study. We selected three typical lesions examined by RCM, and then recorded the results. In the patients with the tinea manus and pedis, mycelium in stratum corneum was found by the RCM in 14 of 22 patients (14/22; 63.64%). In the patients with the tinea cruris, mycelium in stratum corneum was found by the RCM in 19 of 23 patients (19/23; 82.61%). RCM seems to be useful for microscopic evaluation of mycelium features and may have a scientific value in study of superficial cutaneous fungal infections.
传统的皮肤癣菌感染诊断测试目前需要进行皮肤刮片进行光学显微镜检查和/或真菌培养或皮肤活检。免疫荧光显微镜检查也可以用钙荧光素染色。所有这些测试都需要花费时间，需要等待结果，而且具有侵入性。本研究旨在定义浅表皮肤真菌感染的体内反射共聚焦显微镜 (RCM) 特征，并分析与显微镜检查的一致性。总共纳入了 45 名根据显微镜检查阳性结果诊断为浅表皮肤真菌感染的患者。我们选择了三个通过 RCM 检查的典型病变，并记录了结果。在足癣患者中，22 名患者中的 14 名（14/22；63.64%）通过 RCM 发现了角质层中的菌丝。在股癣患者中，23 名患者中的 19 名（19/23；82.61%）通过 RCM 发现了角质层中的菌丝。RCM 似乎可用于微观评估菌丝特征，并且在浅表皮肤真菌感染的研究中可能具有科学价值。
