Dipartimento di Scienze Chirurgiche, Università Cattolica del Sacro Cuore, Rome, Italy.
J Exp Clin Cancer Res. 2012 Sep 11;31(1):71. doi: 10.1186/1756-9966-31-71.
Expression levels of CD133, a cancer stem cell marker, and of the α-subunit of the dystroglycan (α-DG) complex, have been previously reported to be altered in colorectal cancers.
Expression levels of CD133 and α-DG were assessed by immunohistochemistry in a series of colon cancers and their prognostic significance was evaluated.
Scattered cells positive for CD133 were rarely detected at the bases of the crypts in normal colonic mucosa while in cancer cells the median percentage of positive cells was 5% (range 0-80). A significant correlation was observed with pT parameter and tumor stage but not with tumor grade and N status. Recurrence and death from disease were significantly more frequent in CD133-high expressing tumors and Kaplan-Meier curves showed a significant separation between high vs low expressor groups for both disease-free (p = 0.002) and overall (p = 0.008) survival.Expression of α-DG was reduced in a significant fraction of tumors but low α-DG staining did not correlate with any of the classical clinical-pathological parameters. Recurrence and death from the disease were significantly more frequent in α-DG-low expressing tumors and Kaplan-Meier curves showed a significant separation between high vs low expressor tumors for both disease-free (p = 0.02) and overall (p = 0.02) survival. Increased expression of CD133, but not loss of α-DG, confirmed to be an independent prognostic parameters at a multivariate analysis associated with an increased risk of recurrence (RR = 2.4; p = 0.002) and death (RR = 2.3; p = 0.003).
Loss of α-DG and increased CD133 expression are frequent events in human colon cancer and evaluation of CD133 expression could help to identify high-risk colon cancer patients.
CD133，一种癌症干细胞标志物，以及 dystroglycan（α-DG）复合物的α-亚基的表达水平，先前已在结直肠癌中报道发生改变。
通过免疫组织化学在一系列结肠癌中评估 CD133 和 α-DG 的表达水平，并评估其预后意义。
在正常结肠黏膜的隐窝底部很少检测到 CD133 阳性的散在细胞，而在癌细胞中，阳性细胞的中位数百分比为 5%（范围 0-80%）。与 pT 参数和肿瘤分期呈显著相关性，但与肿瘤分级和 N 状态无关。CD133 高表达肿瘤的复发和疾病死亡的频率显著更高，Kaplan-Meier 曲线显示无病生存率（p=0.002）和总生存率（p=0.008）高表达组与低表达组之间有显著分离。α-DG 在显著比例的肿瘤中表达减少，但低 α-DG 染色与任何经典的临床病理参数均无相关性。α-DG 低表达肿瘤的复发和疾病死亡频率显著更高，Kaplan-Meier 曲线显示无病生存率（p=0.02）和总生存率（p=0.02）高表达组与低表达组之间有显著分离。多变量分析证实 CD133 表达增加而不是 α-DG 丢失是独立的预后参数，与复发风险增加（RR=2.4；p=0.002）和死亡风险增加（RR=2.3；p=0.003）相关。
α-DG 的缺失和 CD133 表达的增加是人类结肠癌中常见的事件，评估 CD133 表达可能有助于识别高风险的结肠癌患者。
