Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Shandong University Qilu Hospital, Jinan, Shandong, PR China.
Oncol Rep. 2012 Dec;28(6):2195-9. doi: 10.3892/or.2012.2023. Epub 2012 Sep 11.
Programmed cell death 5 (PDCD5) is a novel apoptosis-related gene with potent antitumor effects which can interact with the histone acetyltransferase Tip60 and induce DNA damage-induced apoptosis. Reduced PDCD5 expression has been found in a few types of human tumors and is also associated with the progression and prognosis of the tumors. However, the expression status and clinical significance of PDCD5 in gastrointestinal stromal tumors has not yet been analyzed. In our study, we examined PDCD5 expression in 63 tumor samples of gastrointestinal stroma at both mRNA and protein levels by RT-PCR, western blotting and immunohistochemistry. We found that 57% (16/28) of the tumor samples had a decreased PDCD5 expression at the mRNA level and 53% (35/66) of the samples were found to have decreased PDCD5 expression at the protein level, whereas PDCD5 was highly expressed in all adjacent normal gastrointestinal tissues at the mRNA or protein level. Moreover, decreased PDCD5 expression was significantly associated with clinicopathological characteristics including tumor size and mitosis. Our results suggest that PDCD5 expression plays a significant role in the malignant progression of human gastrointestinal stromal tumors and may be a key inhibitory factor.
程序性细胞死亡因子 5（PDCD5）是一种新型的与细胞凋亡相关的基因，具有很强的抗肿瘤作用，能够与组蛋白乙酰转移酶 Tip60 相互作用，诱导 DNA 损伤诱导的细胞凋亡。已经发现，在几种类型的人类肿瘤中，PDCD5 的表达降低，并且与肿瘤的进展和预后相关。然而，PDCD5 在胃肠道间质瘤中的表达状态和临床意义尚未被分析。在我们的研究中，我们通过 RT-PCR、western blot 和免疫组织化学检测了 63 例胃肠道间质瘤肿瘤样本中 PDCD5 的表达，同时检测了其在 mRNA 和蛋白水平上的表达。我们发现，57%（16/28）的肿瘤样本在 mRNA 水平上表达降低，53%（35/66）的样本在蛋白水平上表达降低，而 PDCD5 在所有相邻的正常胃肠道组织中均高度表达，无论是在 mRNA 水平还是蛋白水平。此外，PDCD5 表达降低与临床病理特征显著相关，包括肿瘤大小和有丝分裂。我们的结果表明，PDCD5 表达在人类胃肠道间质瘤的恶性进展中起着重要作用，可能是一个关键的抑制因子。
