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负载于含密花角蕨提取物的多孔明胶支架上的自体骨髓基质细胞用于骨修复。

Autologous bone marrow stromal cells loaded onto porous gelatin scaffolds containing Drynaria fortunei extract for bone repair.

机构信息

Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan.

出版信息

J Biomed Mater Res A. 2013 Apr;101(4):954-62. doi: 10.1002/jbm.a.34397. Epub 2012 Sep 10.

Abstract

GGT-GSB composite was prepared by mixing a biodegradable GGT composite containing genipin-crosslinked gelatin and β-tricalcium phosphate with Gu-Sui-Bu extract (GSB) (Drynaria fortunei (Kunze) J. Sm.), a traditional Chinese medicine. Then, porous GGT and GGT-GSB scaffolds were fabricated using a salt-leaching method. The GGT and GGT-GSB scaffolds thus obtained had a macroporous structure and high porosity. Rabbit bone marrow stromal cells (BMSCs) were seeded onto GGT and GGT-GSB scaffolds. The biological response of rabbit calvarial bone to these scaffolds was considered to evaluate the potential of the scaffolds for use in bone tissue engineering. After 8 weeks of implantation, each scaffold induced new bone formation at a cranial bone defect, as was verified by X-ray microradiography. The BMSC-seeded GGT-GSB scaffolds induced more new bone formation than the BMSC-seeded GGT and acellular scaffolds. These observations suggest that an autologous BMSCs-seeded porous GGT-GSB scaffold can be adopted in bone engineering in vivo and has great potential for regenerating defective bone tissue.

摘要

GGT-GSB 复合材料是通过将含有京尼平交联明胶和β-磷酸三钙的可生物降解 GGT 复合材料与中药骨碎补提取物(GSB)(骨碎补(Kunze)J. Sm.)混合制备的。然后,使用盐浸出法制备多孔 GGT 和 GGT-GSB 支架。由此获得的 GGT 和 GGT-GSB 支架具有大孔结构和高孔隙率。将兔骨髓基质细胞(BMSCs)接种到 GGT 和 GGT-GSB 支架上。考虑到支架在骨组织工程中的潜在应用,评估了兔颅骨对这些支架的生物反应。植入 8 周后,通过 X 射线微射线照相术证实,每个支架在颅骨缺损处诱导新骨形成。与 BMSC 接种的 GGT 和无细胞支架相比,BMSC 接种的 GGT-GSB 支架诱导更多的新骨形成。这些观察结果表明,自体 BMSCs 接种的多孔 GGT-GSB 支架可用于体内骨工程,并具有再生缺损骨组织的巨大潜力。

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