Interdisciplinary Division of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region.
J Neurosci Methods. 2012 Sep 30;210(2):272-80. doi: 10.1016/j.jneumeth.2012.07.024. Epub 2012 Aug 9.
This study investigated the blood flow velocity changes in seven major arteries in rat brain before and after intracerebral hemorrhage (ICH) using high frequency transcranial Doppler (TCD) ultrasonography (13-4 MHz). Eighteen adult Sprague Dawley rats received either the collagenase-injection surgery (ICH, n=12) or the saline-injection surgery (control, n=6) after baseline TCD flow velocity values were recorded. The TCD flow velocity changes were measured at 0.5h after the surgery and daily for the following 8 days in seven major cerebral arteries, including bilateral internal carotid arteries, bilateral middle cerebral arteries (MCAs), bilateral posterior cerebral arteries (PCAs), and basilar artery. The results showed a significant decrease of TCD flow velocity in the right MCA at 0.5 h and 24 h after the collagenase-injection surgery, and in the right PCA at 0.5 h in the ICH group rats. The TCD flow velocities in these two arteries gradually increased and then returned to the baseline values in the following days. The control group rats did not show significant changes in TCD flow velocity in all monitored arteries. This study demonstrates the feasibility and reliability of monitoring TCD flow velocity in cerebral arteries using ultrasonography technique in a rat ICH model. The results of this study extend our knowledge in the cerebrovascular changes during intracranial hemorrhage and suggest a possibility of clinical application of TCD ultrasonography in studying the dynamic cerebral circulation after strokes. Moreover, this method could be extensively applied in further studies using potential neuroprotective treatments that affect the cerebral dynamics in the intracerebral hemorrhage.
本研究采用高频经颅多普勒超声（TCD）（13-4MHz）检测脑出血（ICH）前后大鼠脑内 7 条主要动脉的血流速度变化。18 只成年 Sprague Dawley 大鼠接受胶原酶注射手术（ICH，n=12）或生理盐水注射手术（对照，n=6），记录基线 TCD 血流速度值后。在手术后 0.5 小时测量 TCD 血流速度变化，并在随后的 8 天内每天测量 7 条主要脑动脉（双侧颈内动脉、双侧大脑中动脉、双侧大脑后动脉和基底动脉）的 TCD 血流速度。结果显示，ICH 组大鼠右侧大脑中动脉在手术后 0.5 小时和 24 小时以及右侧大脑后动脉在手术后 0.5 小时 TCD 血流速度明显下降。这两条动脉的 TCD 血流速度逐渐增加，随后在随后的几天内恢复到基线值。对照组大鼠在所有监测的动脉中 TCD 血流速度均无明显变化。本研究证明了超声技术监测 ICH 大鼠脑动脉 TCD 血流速度的可行性和可靠性。本研究结果扩展了我们对颅内出血期间脑血管变化的认识，并提示 TCD 超声在研究中风后动态脑循环中的临床应用可能性。此外，这种方法可以广泛应用于进一步研究，研究影响脑出血后大脑动力学的潜在神经保护治疗。
