Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China.
Talanta. 2012 Sep 15;99:959-65. doi: 10.1016/j.talanta.2012.07.066. Epub 2012 Aug 2.
This study describes the synthesis of novel molecularly imprinted polymers based on multi-walled carbon nanotubes (MWNTs@MIPs) using oleanolic acid as the template, 4-vinylpyridine as the functional monomer and divinylbenzene as the cross-linker by heat-induced polymerization. The MWNTs@MIPs were characterized with Fourier transform infrared (FT-IR) spectrometry and scanning electron microscopy (SEM). The adsorption process of the MWNTs@MIPs towards oleanolic acid was investigated in detail. The properties of MWNTs@MIPs for solid-phase extraction (SPE) were also evaluated. The results demonstrated the good imprinting effect and the comparable selectivity of MWNTs@MIPs. The optimized molecularly imprinted solid-phase extraction (MISPE) procedure was applied to extract oleanolic acid from the extracts of the roots of kiwi fruit samples. The recoveries of spiked oleanolic acid in kiwi fruit samples were in the range of 84-92.6% with relative standard deviations below 5%, and its limit of detection reached 2.56 μg L(-1).
本研究描述了以齐墩果酸为模板、4-乙烯吡啶为功能单体、二乙烯基苯为交联剂，通过热引发聚合合成新型分子印迹聚合物（MWNTs@MIPs）。采用傅里叶变换红外光谱（FT-IR）和扫描电子显微镜（SEM）对 MWNTs@MIPs 进行了表征。详细研究了 MWNTs@MIPs 对齐墩果酸的吸附过程。还评价了 MWNTs@MIPs 用于固相萃取（SPE）的性能。结果表明，MWNTs@MIPs 具有良好的印迹效果和可比的选择性。优化后的分子印迹固相萃取（MISPE）程序用于从猕猴桃根提取物中提取齐墩果酸。猕猴桃样品中添加齐墩果酸的回收率在 84-92.6%之间，相对标准偏差低于 5%，检测限达到 2.56μg/L。
