罗氏沼虾幼体对不同剂量高、低毒力白斑综合征病毒（WSSV）毒株的易感性。

Susceptibility of juvenile Macrobrachium rosenbergii to different doses of high and low virulence strains of white spot syndrome virus (WSSV).

作者信息

Corteel Mathias, Dantas-Lima João J, Tuan Vo Van, Thuong Khuong Van, Wille Mathieu, Alday-Sanz Victoria, Pensaert Maurice B, Sorgeloos Patrick, Nauwynck Hans J

机构信息

Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.

出版信息

Dis Aquat Organ. 2012 Sep 12;100(3):211-8. doi: 10.3354/dao02496.

DOI:10.3354/dao02496

PMID:22968789

Abstract

As some literature on the susceptibility of different life stages of Macrobrachium rosenbergii to white spot syndrome virus (WSSV) is conflicting, the pathogenesis, infectivity and pathogenicity of 2 WSSV strains (Thai-1 and Viet) were investigated here in juveniles using conditions standardized for Penaeus vannamei. As with P. vannamei, juvenile M. rosenbergii (2 to 5 g) injected with a low dose of WSSV-Thai-1 or a high dose of WSSV-Viet developed comparable clinical pathology and numbers of infected cells within 1 to 2 d post-infection. In contrast, a low dose of WSSV-Viet capable of causing mortality in P. vannamei resulted in no detectable infection in M. rosenbergii. Mean prawn infectious dose 50% endpoints (PID₅₀ ml⁻¹) determined in M. rosenbergii were in the order of 100-fold higher for WSSV-Thai-1 (105.3 ± 0.4 PID₅₀ ml⁻¹) than for WSSV-Viet (103.2 ± 0.2 PID₅₀ ml⁻¹), with each of these being about 20-fold and 400-fold lower, respectively, than found previously in P. vannamei. The median lethal dose (LD₅₀ ml⁻¹) determined in M. rosenbergii was also far higher (~1000-fold) for WSSV-Thai-1 (105.4 ± 0.4 LD₅₀ ml⁻¹) than for WSSV-Viet (102.3 ± 0.3 LD₅₀ ml⁻¹). Based on these data, it is clear that juvenile M. rosenbergii are susceptible to WSSV infection, disease and mortality. In comparison to P. vannamei, however, juvenile M. rosenbergii appear more capable of resisting infection and disease, particularly in the case of a WSSV strain with lower apparent virulence.

摘要

由于一些关于罗氏沼虾不同生命阶段对白斑综合征病毒（WSSV）易感性的文献存在矛盾之处，本研究采用南美白对虾标准化条件，对2种WSSV毒株（泰国-1株和越南株）在罗氏沼虾幼体中的发病机制、感染性和致病性进行了研究。与南美白对虾一样，注射低剂量WSSV-泰国-1株或高剂量WSSV-越南株的罗氏沼虾幼体（2至5克）在感染后1至2天内出现了类似的临床病理变化和感染细胞数量。相比之下，能导致南美白对虾死亡的低剂量WSSV-越南株在罗氏沼虾中未检测到感染。在罗氏沼虾中测定的半数对虾感染剂量50%终点值（PID₅₀ ml⁻¹），WSSV-泰国-1株（105.3±0.4 PID₅₀ ml⁻¹）比WSSV-越南株（103.2±0.2 PID₅₀ ml⁻¹）高约100倍，且这两个值分别比之前在南美白对虾中发现的值低约20倍和400倍。在罗氏沼虾中测定的半数致死剂量（LD₅₀ ml⁻¹），WSSV-泰国-1株（105.4±0.4 LD₅₀ ml⁻¹）也比WSSV-越南株（102.3±0.3 LD₅₀ ml⁻¹）高得多（约1000倍）。基于这些数据，很明显罗氏沼虾幼体易受WSSV感染、发病和死亡。然而，与南美白对虾相比，罗氏沼虾幼体似乎更能抵抗感染和疾病，特别是在WSSV毒株毒力较低的情况下。