Department of Pharmacology, Wonkwang University School of Medicine, Jeonbuk 570-749, South Korea.
World J Gastroenterol. 2012 Sep 7;18(33):4570-7. doi: 10.3748/wjg.v18.i33.4570.
To investigate whether the combined methods of unilateral thyroparathyroidectomy (TPX) and subdiaphragmatic vagotomy (VAX) can be adapted for rats and used as a reliable method to produce a rat model of long-term reduction of gastrointestinal (GI) motor function.
Male Sprague-Dawley rats were randomly divided into 3 groups, normal, sham-operated and unilateral TPX plus VAX. The TPX plus VAX rats received VAX 7 d after application of TPX, and dietary intake and fecal output were then measured daily for 1 wk. After completion of the experiments, gastric emptying and small bowel transit were measured in vivo, and the contractile responses of colonic strips to excitatory and inhibitory neurotransmitters were estimated using isometric force transducers in vitro.
In comparison with normal and sham-operated rats, rats which received unilateral TPX plus VAX showed a significant decrease in body weight and in fecal pellet number and weight throughout the entire week. Application of TPX plus VAX to rats markedly delayed gastric emptying and small bowel transit. In TPX plus VAX rats, the longitudinal muscles of the proximal colon showed a significant reduction in contractile responses to acetylcholine (5 × 10(-6) mol/L), and a dramatic attenuation of contractile responses was also observed in both the longitudinal and circular muscles of the distal colon. However, the spontaneous contractility of the colonic strips from TPX plus VAX rats was not significantly affected by treatment with N-nitro-L-arginine-methyl ester (0.1 mol/L).
The results indicate that unilateral TPX plus VAX reduced the motor function of the GI tract in rats, and the reduced gut motility is likely mediated, at least in part, by inhibition of the excitatory neurotransmitter system.
探讨单侧甲状腺切除术（TPX）联合膈下迷走神经切断术（VAX）是否适用于大鼠，并作为一种可靠的方法用于产生长期胃肠道（GI）运动功能减弱的大鼠模型。
雄性 Sprague-Dawley 大鼠随机分为 3 组：正常组、假手术组和单侧 TPX 加 VAX 组。TPX 加 VAX 大鼠在应用 TPX 后 7 天接受 VAX，然后每天测量饮食摄入量和粪便排出量 1 周。实验完成后，在体内测量胃排空和小肠转运，并用等力换能器在体外评估结肠条对兴奋性和抑制性神经递质的收缩反应。
与正常组和假手术组相比，接受单侧 TPX 加 VAX 的大鼠在整个星期内体重和粪便颗粒数和重量明显下降。TPX 加 VAX 应用于大鼠显著延迟胃排空和小肠转运。在 TPX 加 VAX 大鼠中，近端结肠的纵行肌对乙酰胆碱（5×10（-6）mol/L）的收缩反应明显减少，同时观察到远端结肠的纵行和环形肌的收缩反应也明显减弱。然而，TPX 加 VAX 大鼠结肠条的自发性收缩不受 N-硝基-L-精氨酸甲酯（0.1mol/L）处理的显著影响。
结果表明，单侧 TPX 加 VAX 降低了大鼠 GI 道的运动功能，而肠道运动功能减弱可能至少部分通过抑制兴奋性神经递质系统介导。
