González-Lucano Luis R, Muñoz-Valle José F, Ascencio-Cedillo Rafael, Domínguez-Rosales José A, López-Rincón Gonzalo, Del Toro-Arreola Susana, Bueno-Topete Miriam, Daneri-Navarro Adrián, Estrada-Chávez Ciro, Pereira-Suárez Ana L
Departments of Physiology, and.
Exp Ther Med. 2012 Apr;3(4):603-607. doi: 10.3892/etm.2012.464. Epub 2012 Jan 30.
The altered expression of the prolactin receptor (PRLR) has been associated with the development of various types of cancer, particularly breast, prostate and endometrial cancer. However, in laryngeal tumors, the expression of PRLR has not yet been documented. The aim of this study was to determine the expression and localization of PRLR in laryngeal cancer (LC) in comparison with recurrent respiratory papillomatosis (RRP). PRLR expression was analyzed in 48 paraffin-embedded tissues (18 RRP and 30 laryngeal cancer tissues) by immunoperoxidase staining. Furthermore, PRLR expression was evaluated in ten samples from each group by Western blot analysis and quantitative real-time PCR. PRLR was observed in all laryngeal tumors at different intensities. PRLR overexpression was significantly associated (P<0.005) with LC. The staining pattern was homogeneous, mainly cytoplasmic, and confined to the tumor area. We found increased expression of different isoforms in LC in comparison with RRP. Our results suggest a possible role of PRL/PRLR in the development of LC. PRLR may be useful as a target for further investigations in laryngeal tissues.
催乳素受体（PRLR）表达的改变与多种类型癌症的发生发展有关，尤其是乳腺癌、前列腺癌和子宫内膜癌。然而，在喉肿瘤中，PRLR的表达尚未见报道。本研究的目的是确定PRLR在喉癌（LC）中的表达和定位，并与复发性呼吸道乳头状瘤病（RRP）进行比较。通过免疫过氧化物酶染色分析了48例石蜡包埋组织（18例RRP和30例喉癌组织）中PRLR的表达。此外，通过蛋白质免疫印迹分析和定量实时聚合酶链反应对每组的10个样本进行PRLR表达评估。在所有喉肿瘤中均观察到不同强度的PRLR。PRLR过表达与LC显著相关（P<0.005）。染色模式均匀，主要位于细胞质，且局限于肿瘤区域。我们发现与RRP相比，LC中不同异构体的表达增加。我们的结果提示PRL/PRLR在LC发生发展中可能发挥作用。PRLR可能作为喉组织进一步研究的靶点。
