花生四烯酸乙醇胺对人肝癌细胞的抗增殖作用。
Anti-proliferative effects of anandamide in human hepatocellular carcinoma cells.
作者信息
Xie Chengzhi, Liu Guoxing, Liu Jiefeng, Huang Zhao, Wang Fusheng, Lei Xiaohua, Wu Xiaolong, Huang Shengfu, Zhong Dewu, Xu Xundi
机构信息
Department of Hepato-Biliary-Pancreatic Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
出版信息
Oncol Lett. 2012 Sep;4(3):403-407. doi: 10.3892/ol.2012.751. Epub 2012 Jun 11.
In our previous study, we reported that the cannabinoid receptors CB1 and CB2 are overexpressed in human hepatocellular carcinoma (HCC) tissues. Recently, the antitumor potential of the endogenous cannabinoid anandamide (AEA) has also been addressed. The present study was conducted to investigate the anti-proliferative effects of AEA in HCC cells. The human HCC cell line Huh7 was used. Cell proliferation was measured by MTT assay and flow cytometry. Apoptotic analysis was investigated by TUNEL assay. Real-time PCR and western blot analysis were used to analyze the expression of relevant molecules. The results of this study demonstrated that AEA inhibited the proliferation of Huh7 cells, resulted in G1 cell cycle arrest and induced apoptosis. Furthermore, downregulation of CDK4 and upregulation of p21 and Bak by AEA were observed. This study defines the anti-proliferative effects of anandamide in HCC cells and suggests that AEA has therapeutic potential in the management of HCC patients.
在我们之前的研究中，我们报道大麻素受体CB1和CB2在人类肝细胞癌（HCC）组织中过表达。最近，内源性大麻素花生四烯乙醇胺（AEA）的抗肿瘤潜力也已得到探讨。本研究旨在调查AEA对肝癌细胞的抗增殖作用。使用了人类肝癌细胞系Huh7。通过MTT法和流式细胞术测量细胞增殖。通过TUNEL法进行凋亡分析。采用实时PCR和蛋白质印迹分析来分析相关分子的表达。本研究结果表明，AEA抑制Huh7细胞的增殖，导致G1期细胞周期停滞并诱导凋亡。此外，观察到AEA使CDK4下调，p21和Bak上调。本研究明确了花生四烯乙醇胺对肝癌细胞的抗增殖作用，并表明AEA在肝癌患者的治疗中具有潜在应用价值。
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