Faculty of Science and Arts, Chemistry Department, Adnan Menderes University, Aydin, Turkey.
Appl Biochem Biotechnol. 2012 Nov;168(6):1528-39. doi: 10.1007/s12010-012-9875-5. Epub 2012 Sep 13.
The purification of immunoglobulin G (IgG) from human plasma was performed by using a novel metal-chelated adsorbent with nano size. The non-porous nanoparticles were produced by surfactant free emulsion polymerization of ethylene glycol dimethacrylate (EDMA) and 2-methacryloylamidohistidine (MAH). Then, Cu(II) ions were chelated on the nanoparticles. The nano-poly(EDMA-MAH) nanoparticles were characterized by Fourier transform infrared, scanning electron microscope, atomic force microscope and elemental analysis. The non-porous nanoparticles were spherical form and have 100-250 nm size distribution. The maximum IgG adsorption capacity of the Cu(II) chelated nanoparticles was found to be 463 mg/g polymer at pH 7.0 in HEPES buffer. Desorption of IgG was performed by 1.0 M NaCl and desorption rate was found to be 97 %. IgG was obtained from human plasma with purity of 94 % (up to 578 mg/g polymer). The non-porous nanoparticles allowed one-step purification of IgG from human plasma.
采用新型纳米尺寸的金属螯合吸附剂从人血浆中纯化免疫球蛋白 G（IgG）。通过无表面活性剂的乙二醇二甲基丙烯酸酯（EDMA）和 2-丙烯酰氨基组氨酸（MAH）的乳液聚合制备了无孔纳米颗粒。然后，将 Cu(II)离子螯合在纳米颗粒上。通过傅里叶变换红外光谱、扫描电子显微镜、原子力显微镜和元素分析对纳米聚（EDMA-MAH）纳米颗粒进行了表征。无孔纳米颗粒呈球形，粒径分布为 100-250nm。在 pH 7.0 的 HEPES 缓冲液中，Cu(II)螯合纳米颗粒的最大 IgG 吸附容量为 463mg/g 聚合物。通过 1.0M NaCl 进行 IgG 的洗脱，洗脱率为 97%。从人血浆中获得纯度为 94%（高达 578mg/g 聚合物）的 IgG。无孔纳米颗粒允许一步法从人血浆中纯化 IgG。
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