Politecnico di Torino, Department of Applied Science and Technology, Corso Duca degli Abruzzi 24, I-10129 Torino, Italy.
Biomed Mater. 2012 Oct;7(5):054102. doi: 10.1088/1748-6041/7/5/054102. Epub 2012 Sep 12.
Grafting of the biomaterial surfaces with biomolecules is nowadays a challenging research field for prosthetic and bone tissue engineering applications. On the other hand, very few research works investigate the effect of the sterilization processes on the properties of functionalized biomaterials. In this study, the effects of different sterilization techniques (e.g. gamma and electron beam irradiation, ethylene oxide) on the enzymatic activity of bioactive glasses and Ti6Al4V grafted with alkaline phosphatase (ALP) have been analyzed. Sterility maintenance and in vitro bioactivity of the sterilized surfaces have also been investigated. Finally the effect of packaging and storage conditions has been considered.
目前，将生物分子嫁接到生物材料表面是义肢和骨组织工程应用领域极具挑战性的研究领域。另一方面，很少有研究工作探讨灭菌过程对功能化生物材料性能的影响。在这项研究中，分析了不同灭菌技术（例如γ射线和电子束辐照、环氧乙烷）对碱性磷酸酶（ALP）接枝的生物活性玻璃和 Ti6Al4V 的酶活性的影响。还研究了灭菌表面的无菌维持和体外生物活性。最后还考虑了包装和储存条件的影响。
