Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai 264003, PR China.
Anal Chim Acta. 2012 Oct 9;746:63-9. doi: 10.1016/j.aca.2012.08.017. Epub 2012 Aug 23.
A novel electrochemical biosensor based on functionalized polypyrrole (PPy) nanotube arrays modified with a tripeptide (Gly-Gly-His) proved to be highly effective for electrochemical analysis of copper ions (Cu(2+)). The vertically oriented PPy nanotube arrays were electropolymerized by using modified zinc oxide (ZnO) nanowire arrays as templates which were electrodeposited on indium-tin oxide (ITO) coated glass substrates. The electrodes were functionalized by appending pyrrole-α-carboxylic acid onto the surface of polypyrrole nanotube arrays by electrochemical polymerization. The carboxylic groups of the polymer were covalently coupled with the amine groups of the tripeptide, and its structural features were confirmed by attenuated total reflection infrared (ATR-IR) spectroscopy. The tripeptide modified PPy nanotube arrays electrode was used for the electrochemical analysis of various trace copper ions by square wave voltammetry. The electrode was found to be highly sensitive and selective to Cu(2+) in the range of 0.1-30 μM. Furthermore, the developed biosensor exhibited a high stability and reproducibility, despite the repeated use of the biosensor electrode.
一种基于功能化聚吡咯（PPy）纳米管阵列的新型电化学生物传感器，该纳米管阵列用三肽（甘氨酰-甘氨酰-组氨酸）修饰，被证明对铜离子（Cu(2+)）的电化学分析非常有效。垂直定向的 PPy 纳米管阵列通过使用改性氧化锌（ZnO）纳米线阵列作为模板进行电化学聚合来制备，这些纳米线阵列被电沉积在铟锡氧化物（ITO）涂覆的玻璃基底上。通过电化学聚合将吡咯-α-羧酸附加到聚吡咯纳米管阵列的表面上来对电极进行功能化。聚合物的羧酸基团通过共价键与三肽的氨基结合，其结构特征通过衰减全反射红外（ATR-IR）光谱得到证实。该三肽修饰的 PPy 纳米管阵列电极通过方波伏安法用于各种痕量铜离子的电化学分析。该电极对 0.1-30 μM 范围内的 Cu(2+)具有高灵敏度和选择性。此外，尽管重复使用生物传感器电极，该生物传感器仍表现出高稳定性和重现性。
