扶正排毒片对32例无症状HIV携带者肿瘤坏死因子-α及新蝶呤的影响

[Effects of fuzheng paidu tablet on tumor necrosis factor-alpha and neopterin of thirty-two asymptomatic HIV carriers].

作者信息

Wang Dan-Ni, Jiang Feng, Guo Hui-Jun

机构信息

Clinical Research Center for AIDS, First Hospital Affiliated to Henan College of Traditional Chinese Medicine, Zhengzhou.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 Jun;32(6):744-7.

PMID:22978092

Abstract

OBJECTIVE

To explore the effects of Fuzheng Paidu Tablet (FPT) on serum levels of tumor necrosis factor-alpha (TNF-alpha) and neopterin (NPI) in patients with asymptomatic human immunodeficiency virus (HIV) carriers.

METHODS

Using flow cytometry detection technology, CD T lymphocyte in anticoagulant blood sample of 32 asymptomatic HIV infection patients who were taking FPT for 6 months was detected and compared with before treatment. The serum levels of TNF-alpha and NPI were determined using ELISA method and compared with 22 healthy volunteers.

RESULTS

After 6 months of treatment by FPT, the CD4+ T lymphocyte of asymptomatic HIV carriers increased from (368.63 +/- 111.54)/mm3 to (412.72 +/- 159.63)/mm3. Before treatment the serum levels of TNF-alpha [(20.05 +/- 13.08) nmol/L] and NPI [(9.55 +/- 2.52) nmol/L] were obviously higher than those of the healthy volunteers [(12.20 +/- 4.07) nmol/L and (5.91 +/- 1.43) nmol/L] (P < 0.05). After 6 months of treatment by FPT, they were lower after treatment than before treatment (P < 0.05). But there was no statistical difference in the serum TNF-alpha level [(11.06 +/- 4.71) nmol/L] when compared with the healthy volunteers group (P > 0.05). But the serum NPI level [(8.08 +/- 2.13) nmol/L] was still higher than that of the healthy volunteers group (P < 0.05).

CONCLUSIONS

One of the pathological factors for asymptomatic HIV infection is abnormal immune activation represented by increased serum levels of TNF-alpha and NPI. FPT could lower the serum levels of TNF-alpha and NPI in asymptomatic HIV infection patients, which was one of its possible mechanisms for its efficacy.

摘要

目的

探讨扶正排毒片（FPT）对无症状人类免疫缺陷病毒（HIV）携带者血清肿瘤坏死因子-α（TNF-α）和新蝶呤（NPI）水平的影响。

方法

采用流式细胞术检测技术，对32例服用FPT 6个月的无症状HIV感染患者抗凝血样中的CD⁺T淋巴细胞进行检测，并与治疗前进行比较。采用酶联免疫吸附测定（ELISA）法测定血清TNF-α和NPI水平，并与22名健康志愿者进行比较。

结果

FPT治疗6个月后，无症状HIV携带者的CD4⁺T淋巴细胞从（368.63±111.54）/mm³增至（412.72±159.63）/mm³。治疗前血清TNF-α水平[（20.05±13.08）nmol/L]和NPI水平[（9.55±2.52）nmol/L]明显高于健康志愿者[（12.20±4.07）nmol/L和（5.91±1.43）nmol/L]（P<0.05）。FPT治疗6个月后，治疗后水平低于治疗前（P<0.05）。但与健康志愿者组比较，血清TNF-α水平[（11.06±4.71）nmol/L]无统计学差异（P>0.05）。但血清NPI水平[（8.08±2.13）nmol/L]仍高于健康志愿者组（P<0.05）。