[白细胞介素-17对从BALB/c小鼠分离的心脏成纤维细胞中I/III型胶原蛋白表达的影响]
[Effect of IL-17 on collagen I/III expression in cardiac fibroblasts isolated from BALB/c mice].
作者信息
Su Zhao-liang, Wang Ying-mei, Liu Yan-fang, Wang Ting, Tian Sha-sha, Zhang Pan, Ji Xiao-yun, Ma Ke, Xu Hua-xi
机构信息
Institute of Immunology, Jiangsu University, Zhenjiang, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Sep;28(9):897-8, 902.
AIM
To investigate the effect of IL-17 on the expression of collagen I/III in cardiac fibroblasts and analyze its molecular mechanism.
METHODS
Cardiac fibroblasts were isolated from 7-14-day-old BALB/c mice and cultured in DMEM with 10% fetal bovine serum (FBS). The cells were collected after IL-17 treatment for 0, 24, 48, 72 h. IL-17 receptors on cardiac fibroblasts were detected by PCR; the collagen I/III expression was analyzed by immunofluorescence; the PKCβ, Erk1/2, NF-κB phosphorylation were investigated by Western blotting.
RESULTS
IL-17RA/C was expressed on cardiac fibroblasts; after 24 h of IL-17 stimulation, the collagen I/III expression obviously increased; Western blotting showed that PKCβ, Erk1/2 and NF-κB were phosphorylated on 30, 45, 45 min, respectively.
CONCLUSION
IL-17 could induce the expression of collagen I/III in cardiac fibroblasts, which might be related with PKCβ-ERK1/2-NF-κB phosphorylation.
目的
研究白细胞介素-17（IL-17）对心脏成纤维细胞中I/III型胶原蛋白表达的影响，并分析其分子机制。
方法
从7至14日龄的BALB/c小鼠中分离出心脏成纤维细胞，在含有10%胎牛血清（FBS）的DMEM培养基中培养。在IL-17处理0、24、48、72小时后收集细胞。通过PCR检测心脏成纤维细胞上的IL-17受体；通过免疫荧光分析I/III型胶原蛋白的表达；通过蛋白质印迹法研究蛋白激酶Cβ（PKCβ）、细胞外信号调节激酶1/2（Erk1/2）、核因子κB（NF-κB）的磷酸化情况。
结果
心脏成纤维细胞上表达IL-17RA/C；IL-17刺激24小时后，I/III型胶原蛋白表达明显增加；蛋白质印迹法显示PKCβ、Erk1/2和NF-κB分别在30、45、45分钟时发生磷酸化。
结论
IL-17可诱导心脏成纤维细胞中I/III型胶原蛋白的表达，这可能与PKCβ-ERK1/2-NF-κB磷酸化有关。
Zotero 插件