中国分离的柑橘衰退病毒株系的基因型通过多个分子标记的序列分析揭示。
The genotypes of citrus tristeza virus isolates from China revealed by sequence analysis of multiple molecular markers.
机构信息
National Key Laboratory of Agromicrobiology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
出版信息
Arch Virol. 2013 Jan;158(1):231-5. doi: 10.1007/s00705-012-1475-8. Epub 2012 Sep 18.
The genotypes of ten citrus tristeza virus (CTV) isolates from central China were determined by examining multiple molecular markers (MMMs) using 11 primer pairs. The results revealed that one isolate contained a single T30 genotype, two isolates contained a single VT genotype, and the other seven isolates were mixtures of two or more genotypes. Sequence analysis of amplified MMMs showed a high genetic diversity in Chinese CTV populations. The genotypes resembling T36, RB and B165 were identified from Chinese CTV isolates for the first time. Our results suggest that genotype assignment of CTV cannot be based solely on the amplification profiles of MMMs, and sequencing of MMMs is required.
利用 11 对引物对来自中国中部的 10 个柑橘衰退病毒（CTV）分离物的多个分子标记（MMMs）进行检测，确定了它们的基因型。结果表明，一个分离物含有单一的 T30 基因型，两个分离物含有单一的 VT 基因型，而其他七个分离物则是两种或更多基因型的混合物。对扩增 MMMs 的序列分析表明，中国 CTV 群体具有很高的遗传多样性。首次从中国 CTV 分离物中鉴定出类似于 T36、RB 和 B165 的基因型。我们的结果表明，对 CTV 的基因型分配不能仅仅基于 MMMs 的扩增谱，还需要对 MMMs 进行测序。
Zotero 插件