Zakharova L A, Surova G S, Timofeev K N
Izv Akad Nauk Ser Biol. 2012 Jul-Aug(4):373-82.
In a natural pond among usual black larvae of the common toad (Bufo bufo L.), a few unusual individuals of red-olive coloring were found out. In both morphs we investigated the melanophores of skin using different methods. The ESR-spectrometric analysis has shown the absence of distinctions between morphs by the amount of melanin. Analysis of total preparations of skin has shown the presence of various kinds of melanophore cells both in the derma and in the epidermis. Among typical melanophores, essentially differing cells appeared (atypical cells). In black morph tadpoles, the number of all kinds of melanophores is significantly greater than in red-olive morphs. It is shown that dark coloring is connected with a considerable number of atypical cells in the epidermis imposed on a dense layer of typical dermal melanophores with dispersed melanin.
在一个普通蟾蜍(Bufo bufo L.)常见黑色幼虫的天然池塘中,发现了几只颜色为红橄榄色的不寻常个体。我们用不同方法研究了这两种形态个体皮肤中的黑素细胞。电子自旋共振光谱分析表明,两种形态在黑色素含量上没有差异。皮肤整体制剂分析表明,真皮和表皮中均存在各种类型的黑素细胞。在典型的黑素细胞中,出现了本质上不同的细胞(非典型细胞)。黑色形态的蝌蚪中,各类黑素细胞的数量明显多于红橄榄色形态。结果表明,深色与表皮中大量的非典型细胞有关,这些非典型细胞叠加在含有分散黑色素的致密典型真皮黑素细胞层之上。